nps Natural Product Sciences Natural Product Sciences 1226-3907 2288-9027 The Korean Society of Pharmacognosy 10.20307/nps.2016.22.4.238 nps-22-238 Original Article Simultaneous Determination of 11 Marker Compounds in Gumiganghwal-tang by HPLC-DAD and LC-MS WeonJin Bae 1 JungYoun Sik 1 RyuGahee 1 YangWoo Seung 1 MaChoong Je 1 2 ∗ Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200–701, Korea Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200–701, Korea ∗Author for correspondence Choong Je Ma, Ph.D. Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea Tel: +82-33-250-6565; E-mail: cjma@kangwon.ac.kr 01 2016 20 01 2016 22 4 238 245 46042016 27052016 04062016 Copyright © 2016 The Korean Society of Pharmacognosy 2016 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract

Gumiganghwal-tang has been used for the treatment of common cold for a long-time. We developed an accurate and sensitive high performance liquid chromatography-diode array detection (HPLC-DAD) and electrospray ionization mass spectrometry method for the simultaneous determination of ferulic acid, baicalin, bergapten, methyl eugenol, glycyrrhizin, oxypeucedanin, wogonin, nodakenin, atractylenolide III, imperatorin, and atractylenolide I in Gumiganghwal-tang samples. The analytes were separated on a Shiseido C18 column (5 µm, 4.6 mm I.D. × 250 mm) with gradient elution with acetonitrile and 0.1% trifluoroacetic acid. Eleven compounds were quantitatively determined by HPLC-DAD and identified by LC-MS data. We also validated this method. The calibration curves of all the compounds showed good linear regression. The limits of detection and the limits of quantification ranged from 0.04 to 0.63 and from 0.12 to 1.92µg/mL, respectively. The relative standard deviation values of intra- and inter-days of this method represented less than 2.9%. The recoveries were found to be in the range of 90.06–107.66%. The developed method has been successfully applied to the analysis of Gumiganghwal-tang samples. The established HPLC method could be used to quality control of Gumiganghwal-tang.

 Simultaneous determination Gumiganghwal-tang HPLC-DAD LC-MS References Jiang W. Y. Trends Pharmacol. Sci. 2005 26 558563 Liang Y. Z. Xie P. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2004 812 5370 Bent S. J. Gen. Intern. Med. 2008 23 854859 Firenzuoli F. Gori L. Evid. Based Complement. Alternat. Med. 2007 4 3740 Moon Y. H. Go J. J. Park J. Y. Kor. J. Pharmacogn. 1999 30 1824 Kim S. J. Jeong H. J. Moon P. D. Lee K. M. Lee H. B. Jung H. J. Jung S. K. Rhee H. K. Yang D. C. Hong S. H. Kim H. M. Biol. Pharm. Bull. 2005 28 233237 Lee M. Y. Seo C. S. Shin I. S. Ha H. Kim J. H. Cho J. W. Huh J. I. Shin H. K. Regul. Toxicol. Pharmacol. 2012 62 553560 Jung H. W. Mahesh R. Park J. H. Boo Y. C. Park K. M. Park Y. K. Int. Immunopharmacol. 2010 10 155162 Jung H. W. Jung J. K. Park Y. K. AsianPac. J. Allergy Immunol. 2011 29 338348 Kang T. J. Lee S. Y. Singh R. P. Agarwal R. Yim D. S. Acta. Oncol. 2009 48 895900 Okuyama E. Hasegawa T. Matsushita T. Fujimoto H. Ishibashi M. Yamazaki M. Chem. Pharm. Bull. 2001 49 154160 Chin Y. W. Jung Y. H. Chae H. S. Yoon K. D. Kim J. Bull. Korean Chem. Soc. 2011 32 21322134 Li S. Han Q. Qiao C. Song J. Lung Cheng C. Xu H. Chin. Med. 2008 3 DOI: 10.1186/1749-8546-3-7 Choi D. W. Kim J. H. Cho S. Y. Kim D. H. Chang S. Y. Toxicology 2002 181–182 581586 Lin S. J. Tseng H. H. Wen K. C. Suen T. T. J. Chromatogr. A 1996 730 1723 Liu Q. Zhao J. Yan L. Yi J. Song J. Zhongguo Zhong Yao Za Zhi 2010 35 708710 Zuo F. Zhou Z. M. Liu M. L. Biol. Pharm. Bull. 2001 24 693697 Jiang P. Shi R. Wang Q. Ma Y. M. Cui H. Y. Liu P. Liu C. H. Biomed. Chromatogr. 2013 27 874881 Samanidou V. Tsagiannidis A. Sarakatsianos I. J. Sep. Sci. 2012 35 608615 Sun Q. Chang L. Ren Y. Cao L. Sun Y. Du Y. Shi X. Wang Q. Zhang L. J. Sep. Sci. 2012 35 28972907 Weon J. B. Yang H. J. Ma J. Y. Ma C. J. J. Nat. Med. 2012 66 510515 Shin I. S. Seo C. S. Lee M. Y. Ha H. K. Huh J. I. Shin H. K. J. Ethnopharmacol. 2012 141 350356 Figures

Chemical structures of the 11 standard compounds of Gumiganghwal-tang.

UV wavelength of the 11 standard compounds of Gumiganghwal-tang.

HPLC chromatogram of standard compounds mixture (A) and Gumiganghwal -tang sample (B). Peaks: (1) ferulic acid, (2) nodakenin, (3) baicalin, (4) bergapten, (5) glycyrrhizin, (6) oxypeucedanin, (7) wogonin, (8) methyl eugenol, (9) atractylenolide III, (10) imperatorin and (11) atractylenolide I.

SIM chromatograms and product ion scan spectra of the 11 marker compounds of Gumiganghwal-tang. Numbers: (1) ferulic acid, (2) nodakenin, (3) baicalin, (4) bergapten, (5) glycyrrhizin, (6) oxypeucedanin, (7) wogonin, (8) methyl eugenol, (9) atractylenolide III, (10) imperatorin and (11) atractylenolide I.