<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" "journalpublishing.dtd">
<?xml-stylesheet type="text/xsl" href="ViewNLM-v2.3.xsl"?>
<article xml:lang="KO" article-type="research-article">

<front>
<journal-meta>
<journal-id journal-id-type="nlm-ta">J Korean Acad Conserv Dent</journal-id>
<journal-id journal-id-type="publisher-id">JKACD</journal-id>
<journal-title>Journal of Korean Academy of Conservative Dentistry</journal-title>
<issn pub-type="ppub">1225-0864</issn>
<issn pub-type="epub">2093-8179</issn>
<publisher>
<publisher-name>The Korean Academy of Conservative Dentistry</publisher-name>
</publisher>
</journal-meta>

<article-meta>

<article-id pub-id-type="doi">10.5395/JKACD.2008.33.1.066</article-id>

<article-categories>
<subj-group>
<subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Evaluation of the influence of apical sizes on the apical sealing ability of the modified continuous wave technique</article-title>
</title-group>

<contrib-group>

<contrib contrib-type="author">
<name>
<surname>Ryu</surname>
<given-names>Muhyun</given-names>
</name>
<xref ref-type="aff" rid="A1"></xref>
</contrib>

<contrib contrib-type="author">
<name>
<surname>Jung</surname>
<given-names>Ilyoung</given-names>
</name>
<xref ref-type="aff" rid="A1"></xref>
</contrib>

<contrib contrib-type="author">
<name>
<surname>Lee</surname>
<given-names>Seungjong</given-names>
</name>
<xref ref-type="aff" rid="A1"></xref>
</contrib>

<contrib contrib-type="author">
<name>
<surname>Shin</surname>
<given-names>Sujung</given-names>
</name>
<xref ref-type="aff" rid="A1"></xref>
</contrib>

<contrib contrib-type="author" corresp="yes">
<name>
<surname>Kim</surname>
<given-names>Euiseong</given-names>
</name>
<xref ref-type="aff" rid="A1"></xref>
</contrib>

</contrib-group>

<aff id="A1">Department of Dentistry, The Graduate School, Yonsei University, Korea.</aff>

<author-notes>
<corresp>Corresponding Author: Euiseong Kim. Department of Conservative Dentistry, College of Dentistry, Yonsei University, 134 Shinchon-Dong, Seodamun-Ku, Seoul, 120-752, Korea. Tel: 82-2-2228-8701, Fax: 82-2-313-7575, <email>andyendo@yuhs.ac</email></corresp>
</author-notes>

<pub-date pub-type="ppub">
<month>01</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="epub">
<day>31</day>
<month>01</month>
<year>2008</year>
</pub-date>
<volume>33</volume>
<issue>1</issue>
<fpage>66</fpage>
<lpage>75</lpage>
<history>
<date date-type="received">
<day>22</day>
<month>10</month>
<year>2007</year>
</date>
<date date-type="rev-recd">
<day>11</day>
<month>12</month>
<year>2007</year>
</date>
<date date-type="accepted">
<day>17</day>
<month>01</month>
<year>2008</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2008 The Korean Academy of Conservative Dentistry</copyright-statement>
<copyright-year>2008</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">
<p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
</license>
</permissions>

<abstract>
  <p>This study examined the influence of the apical sizes on the sealing ability of a root canal filling.</p>
  <p>Thirty-six single rooted teeth with a single canal were divided into 3 groups (n = 12) and instrumented with either the Profile&#x00AE; or LightSpeed&#x00AE; system to achieve three different apical sizes (master apical file [MAF] of &#x0023;25, &#x0023;40, or &#x0023;60). The teeth were filled with gutta percha using a modified continuous wave technique. The level of microleakage was determined by immersing ten teeth from each group into India ink for 1 week followed by clearing with nitric acid, ethyl-alcohol, and methylsalicylate. The microleakage was measured using vernier calipers. The data was analyzed statistically using Kruskal-Wallis one-way ANOVA and a Student-Newman-Keuls Method. Two teeth from each group were sectioned horizontally at 1, 2, 3 and 4 mm from the apex in order to observe a cross section.</p>
  <p>The apical size was significantly (p &#x003C; .05) influenced the level of microleakage. In the Student-Newman-Deuls Method, MAF sizes of &#x0023;25 and &#x0023;40; and MAF sizes of &#x0023;25 and &#x0023;60, respectively showed a statistically significant difference. There was no significant difference between &#x0023;40 and &#x0023;60. In most cross sections, oval-shaped canals were observed, and the irregularity of the internal surface increased with decreasing apical size. There was also an increase in the area of recess, which is the area where the canal space is not filled with either gutta-percha or sealer.</p>
  <p>When the root canals are filled using a modified continuous wave technique, canal filling with more consistent and predictable outcome may be expected as the apical preparation size is increased.</p>
</abstract>

<kwd-group>
<kwd>Apical enlargement</kwd>
<kwd>apical leakage</kwd>
<kwd>apical size</kwd>
<kwd>sealing ability</kwd>
</kwd-group>

</article-meta>
</front>

<back>

    <ref-list>

  <ref id="B1">
    <label>1</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Schilder</surname>
          <given-names>H</given-names>
        </name>
      </person-group>
      <article-title>Filling root canals in three dimensions</article-title>
      <source>Dent Clin North Am</source>
      <year>1967</year>
      <fpage>723</fpage>
      <lpage>744</lpage>
    </nlm-citation>
  </ref>

  <ref id="B2">
    <label>2</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Sj&#x00F6;gren</surname>
          <given-names>U</given-names>
        </name>
        <name>
          <surname>H&#x0103;agglund</surname>
          <given-names>B</given-names>
        </name>
        <name>
          <surname>Sundqvist</surname>
          <given-names>G</given-names>
        </name>
        <name>
          <surname>Wing</surname>
          <given-names>K</given-names>
        </name>
      </person-group>
      <article-title>Factors affecting the long-term results of endodontic treatment</article-title>
      <source>J Endod</source>
      <year>1990</year>
      <volume>16</volume>
      <fpage>498</fpage>
      <lpage>504</lpage>
    </nlm-citation>
  </ref>

  <ref id="B3">
    <label>3</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Rud</surname>
          <given-names>J</given-names>
        </name>
        <name>
          <surname>Andreasen</surname>
          <given-names>JO</given-names>
        </name>
      </person-group>
      <article-title>A study of failures after endodontic surgery by radiographic, histologic, and stereomicroscopic methods</article-title>
      <source>Int J Oral Surg</source>
      <year>1972</year>
      <volume>1</volume>
      <fpage>311</fpage>
      <lpage>328</lpage>
    </nlm-citation>
  </ref>

  <ref id="B4">
    <label>4</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Gutmann</surname>
          <given-names>JL</given-names>
        </name>
      </person-group>
      <article-title>Clinical, radiographic, and histologic perspectives on success and failure in endodontics</article-title>
      <source>Dent Clin North Am</source>
      <year>1992</year>
      <volume>36</volume>
      <fpage>379</fpage>
      <lpage>392</lpage>
    </nlm-citation>
  </ref>

  <ref id="B5">
    <label>5</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Buckley</surname>
          <given-names>M</given-names>
        </name>
        <name>
          <surname>Sp&#x00E1;ngberg</surname>
          <given-names>L</given-names>
        </name>
      </person-group>
      <article-title>The prevalence and technical quality of endodontic treatment in an American subpopulation</article-title>
      <source>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</source>
      <year>1995</year>
      <volume>79</volume>
      <fpage>92</fpage>
      <lpage>100</lpage>
    </nlm-citation>
  </ref>

  <ref id="B6">
    <label>6</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Youngson</surname>
          <given-names>CC</given-names>
        </name>
        <name>
          <surname>Nattress</surname>
          <given-names>BR</given-names>
        </name>
        <name>
          <surname>Manogue</surname>
          <given-names>M</given-names>
        </name>
        <name>
          <surname>Speirs</surname>
          <given-names>AF</given-names>
        </name>
      </person-group>
      <article-title>In vitro radiographic representation of the extent of voids within obturated root canals</article-title>
      <source>Int Endod J</source>
      <year>1995</year>
      <volume>28</volume>
      <fpage>77</fpage>
      <lpage>81</lpage>
    </nlm-citation>
  </ref>

  <ref id="B7">
    <label>7</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Ainley</surname>
          <given-names>JE</given-names>
        </name>
      </person-group>
      <article-title>Fluorometric assay of the apical seal of root canal fillings</article-title>
      <source>Oral Surg Oral Med Oral Pathol</source>
      <year>1970</year>
      <volume>29</volume>
      <fpage>753</fpage>
      <lpage>762</lpage>
    </nlm-citation>
  </ref>

  <ref id="B8">
    <label>8</label>
    <nlm-citation citation-type="book">
      <person-group person-group-type="author">
        <name>
          <surname>Kuttler</surname>
          <given-names>J</given-names>
        </name>
      </person-group>
      <source>Fundamentos de Endo-metaendodoncia Practica</source>
      <year>1980</year>
      <edition>2nd ed</edition>
      <publisher-loc>Mexico</publisher-loc>
      <publisher-name>Mendez Ostero</publisher-name>
      <fpage>8</fpage>
    </nlm-citation>
  </ref>

  <ref id="B9">
    <label>9</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Green</surname>
          <given-names>D</given-names>
        </name>
      </person-group>
      <article-title>A stereo-binocular microscopic study of the root apices and surrounding areas of 100 mandibular molars ; preliminary study</article-title>
      <source>Oral Surg Oral Med Oral Pathol</source>
      <year>1955</year>
      <volume>8</volume>
      <fpage>1298</fpage>
      <lpage>1304</lpage>
    </nlm-citation>
  </ref>

  <ref id="B10">
    <label>10</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Ponce</surname>
          <given-names>EH</given-names>
        </name>
        <name>
          <surname>Vilar Fernandez</surname>
          <given-names>JA</given-names>
        </name>
      </person-group>
      <article-title>The cemento-dentinocanal junction, the apical foramen, and the apical constriction : evaluation by optical microscopy</article-title>
      <source>J Endod</source>
      <year>2003</year>
      <volume>29</volume>
      <fpage>214</fpage>
      <lpage>219</lpage>
    </nlm-citation>
  </ref>

  <ref id="B11">
    <label>11</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Wu</surname>
          <given-names>MK</given-names>
        </name>
        <name>
          <surname>Roris</surname>
          <given-names>A</given-names>
        </name>
        <name>
          <surname>Barkis</surname>
          <given-names>D</given-names>
        </name>
        <name>
          <surname>Wesselink</surname>
          <given-names>PR</given-names>
        </name>
      </person-group>
      <article-title>Prevalence and extent of long oval canals in the apical third</article-title>
      <source>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</source>
      <year>2000</year>
      <volume>89</volume>
      <fpage>739</fpage>
      <lpage>743</lpage>
    </nlm-citation>
  </ref>

  <ref id="B12">
    <label>12</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Buchanan</surname>
          <given-names>LS</given-names>
        </name>
      </person-group>
      <article-title>The standardized-taper root canal preparation : part1. Concepts for variably tapered shaping instruments</article-title>
      <source>Int Endod J</source>
      <year>2000</year>
      <volume>33</volume>
      <fpage>516</fpage>
      <lpage>529</lpage>
    </nlm-citation>
  </ref>

  <ref id="B13">
    <label>13</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Buchanan</surname>
          <given-names>LS</given-names>
        </name>
      </person-group>
      <article-title>The standardized-taper root canal preparation : part2. GT file selection and safe handpiece-driven file use</article-title>
      <source>Int Endod J</source>
      <year>2001</year>
      <volume>34</volume>
      <fpage>63</fpage>
      <lpage>71</lpage>
    </nlm-citation>
  </ref>

  <ref id="B14">
    <label>14</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Buchanan</surname>
          <given-names>LS</given-names>
        </name>
      </person-group>
      <article-title>The standardized-taper root canal preparation : part3. GT file technique in large root canals with small apical diameters</article-title>
      <source>Int Endod J</source>
      <year>2001</year>
      <volume>34</volume>
      <fpage>149</fpage>
      <lpage>156</lpage>
    </nlm-citation>
  </ref>

  <ref id="B15">
    <label>15</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Buchanan</surname>
          <given-names>LS</given-names>
        </name>
      </person-group>
      <article-title>The standardized-taper root canal preparation : part4. GT file technique in large root canals with large apical diameters</article-title>
      <source>Int Endod J</source>
      <year>2001</year>
      <volume>34</volume>
      <fpage>149</fpage>
      <lpage>156</lpage>
    </nlm-citation>
  </ref>

  <ref id="B16">
    <label>16</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Buchanan</surname>
          <given-names>LS</given-names>
        </name>
      </person-group>
      <article-title>The standardized-taper root canal preparation : part5. GT file technique in small root canals</article-title>
      <source>Int Endod J</source>
      <year>2001</year>
      <volume>34</volume>
      <fpage>244</fpage>
      <lpage>249</lpage>
    </nlm-citation>
  </ref>

  <ref id="B17">
    <label>17</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Buchanan</surname>
          <given-names>LS</given-names>
        </name>
      </person-group>
      <article-title>The standardized-taper root canal preparation : part6. GT file technique in abruptly curved canals</article-title>
      <source>Int Endod J</source>
      <year>2001</year>
      <volume>34</volume>
      <fpage>250</fpage>
      <lpage>259</lpage>
    </nlm-citation>
  </ref>

  <ref id="B18">
    <label>18</label>
    <nlm-citation citation-type="book">
      <person-group person-group-type="author">
        <name>
          <surname>Weine</surname>
          <given-names>F</given-names>
        </name>
      </person-group>
      <source>Endodontic therapy</source>
      <year>1972</year>
      <publisher-loc>St. Louis</publisher-loc>
      <publisher-name>C.V. Mosby</publisher-name>
      <fpage>209</fpage>
      <lpage>222</lpage>
    </nlm-citation>
  </ref>

  <ref id="B19">
    <label>19</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Wu</surname>
          <given-names>MK</given-names>
        </name>
        <name>
          <surname>Barkis</surname>
          <given-names>D</given-names>
        </name>
        <name>
          <surname>Roris</surname>
          <given-names>A</given-names>
        </name>
        <name>
          <surname>Wesselink</surname>
          <given-names>PR</given-names>
        </name>
      </person-group>
      <article-title>Does the first file to bind correspond to the diameter of the canal in the apical region?</article-title>
      <source>Int Endod J</source>
      <year>2002</year>
      <volume>35</volume>
      <fpage>264</fpage>
      <lpage>267</lpage>
    </nlm-citation>
  </ref>

  <ref id="B20">
    <label>20</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Strindberg</surname>
          <given-names>LZ</given-names>
        </name>
      </person-group>
      <article-title>The dependence of results of pulp therapy on certain factors : an analytic study based on radiographic and clinical follow-up examination</article-title>
      <source>Acta Odontol Scand</source>
      <year>1956</year>
      <volume>14</volume>
      <supplement>Suppl</supplement>
      <fpage>1</fpage>
    </nlm-citation>
  </ref>

  <ref id="B21">
    <label>21</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Hoskinson</surname>
          <given-names>SE</given-names>
        </name>
        <name>
          <surname>Ng</surname>
          <given-names>YL</given-names>
        </name>
        <name>
          <surname>Hoskinson</surname>
          <given-names>AE</given-names>
        </name>
        <name>
          <surname>Moles</surname>
          <given-names>DR</given-names>
        </name>
        <name>
          <surname>Gulabivala</surname>
          <given-names>K</given-names>
        </name>
      </person-group>
      <article-title>A retrospective comparison of outcome of root canal treatment using two different protocols</article-title>
      <source>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</source>
      <year>2002</year>
      <volume>93</volume>
      <fpage>705</fpage>
      <lpage>715</lpage>
    </nlm-citation>
  </ref>

  <ref id="B22">
    <label>22</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Friedman</surname>
          <given-names>S</given-names>
        </name>
        <name>
          <surname>Abitbol</surname>
          <given-names>S</given-names>
        </name>
        <name>
          <surname>Lawrence</surname>
          <given-names>HP</given-names>
        </name>
      </person-group>
      <article-title>Treatment outcome in endodontics : the Toronto Study. Phase 1 : initial treatment</article-title>
      <source>J Endod</source>
      <year>2003</year>
      <volume>29</volume>
      <fpage>787</fpage>
      <lpage>793</lpage>
    </nlm-citation>
  </ref>

  <ref id="B23">
    <label>23</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Wu</surname>
          <given-names>MK</given-names>
        </name>
        <name>
          <surname>Schwartz</surname>
          <given-names>FB</given-names>
        </name>
        <name>
          <surname>Van des Sluis</surname>
          <given-names>LW</given-names>
        </name>
        <name>
          <surname>Wesselink</surname>
          <given-names>PR</given-names>
        </name>
      </person-group>
      <article-title>The quality of root fillings remaining in mandibular incisors after root-end cavity preparation</article-title>
      <source>Int Endod J</source>
      <year>2001</year>
      <volume>34</volume>
      <fpage>613</fpage>
      <lpage>619</lpage>
    </nlm-citation>
  </ref>

  <ref id="B24">
    <label>24</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Wu</surname>
          <given-names>MK</given-names>
        </name>
        <name>
          <surname>Wesselink</surname>
          <given-names>PR</given-names>
        </name>
      </person-group>
      <article-title>Efficacy of three techniques in cleaning the apical portion of the curved root canals</article-title>
      <source>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</source>
      <year>1995</year>
      <volume>79</volume>
      <fpage>492</fpage>
      <lpage>496</lpage>
    </nlm-citation>
  </ref>

  <ref id="B25">
    <label>25</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Siqueira</surname>
          <given-names>JE</given-names>
        </name>
        <name>
          <surname>Araujo</surname>
          <given-names>MC</given-names>
        </name>
        <name>
          <surname>Garcia</surname>
          <given-names>PF</given-names>
        </name>
        <name>
          <surname>Fraga</surname>
          <given-names>RC</given-names>
        </name>
        <name>
          <surname>Dantas</surname>
          <given-names>CJ</given-names>
        </name>
      </person-group>
      <article-title>Histological evaluation of the effectiveness of five instrumentation techniques for cleaning the apical third of root canals</article-title>
      <source>J Endod</source>
      <year>1997</year>
      <volume>23</volume>
      <fpage>499</fpage>
      <lpage>502</lpage>
    </nlm-citation>
  </ref>

  <ref id="B26">
    <label>26</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Rollison</surname>
          <given-names>S</given-names>
        </name>
        <name>
          <surname>Barnett</surname>
          <given-names>F</given-names>
        </name>
        <name>
          <surname>Stevens</surname>
          <given-names>RM</given-names>
        </name>
      </person-group>
      <article-title>Efficacy of bacterial removal from instrumented root canals in vitro related to instrumentation technique and size</article-title>
      <source>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</source>
      <year>2002</year>
      <volume>94</volume>
      <fpage>366</fpage>
      <lpage>371</lpage>
    </nlm-citation>
  </ref>

  <ref id="B27">
    <label>27</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Card</surname>
          <given-names>SJ</given-names>
        </name>
        <name>
          <surname>Sigurdsson</surname>
          <given-names>A</given-names>
        </name>
        <name>
          <surname>Orstavik</surname>
          <given-names>D</given-names>
        </name>
        <name>
          <surname>Trope</surname>
          <given-names>M</given-names>
        </name>
      </person-group>
      <article-title>The effectiveness of increased apical enlargement in reducing intracanal bacteria</article-title>
      <source>J Endod</source>
      <year>2002</year>
      <volume>28</volume>
      <fpage>779</fpage>
      <lpage>783</lpage>
    </nlm-citation>
  </ref>

  <ref id="B28">
    <label>28</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Tan</surname>
          <given-names>BT</given-names>
        </name>
        <name>
          <surname>Messer</surname>
          <given-names>HH</given-names>
        </name>
      </person-group>
      <article-title>The quality of apical canal preparation using hand and rotary instruments with specific criteria for enlargement based on initial apical file size</article-title>
      <source>J Endod</source>
      <year>2002</year>
      <volume>28</volume>
      <fpage>658</fpage>
      <lpage>664</lpage>
    </nlm-citation>
  </ref>

  <ref id="B29">
    <label>29</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Shuping</surname>
          <given-names>GB</given-names>
        </name>
        <name>
          <surname>&#x00D8;rstavik</surname>
          <given-names>D</given-names>
        </name>
        <name>
          <surname>Sigurdsson</surname>
          <given-names>A</given-names>
        </name>
        <name>
          <surname>Trope</surname>
          <given-names>M</given-names>
        </name>
      </person-group>
      <article-title>Reduction of intracanal bacteria using nickel-titanium rotary instrumentation and various medications</article-title>
      <source>J Endod</source>
      <year>2000</year>
      <volume>26</volume>
      <fpage>751</fpage>
      <lpage>755</lpage>
    </nlm-citation>
  </ref>

  <ref id="B30">
    <label>30</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Sj&#x00F6;gren</surname>
          <given-names>U</given-names>
        </name>
        <name>
          <surname>H&#x0103;gglund</surname>
          <given-names>B</given-names>
        </name>
        <name>
          <surname>Sundqvist</surname>
          <given-names>G</given-names>
        </name>
        <name>
          <surname>Wing</surname>
          <given-names>K</given-names>
        </name>
      </person-group>
      <article-title>Factors affecting the long-term results of endodontic treatment</article-title>
      <source>J Endod</source>
      <year>1990</year>
      <volume>16</volume>
      <fpage>498</fpage>
      <lpage>504</lpage>
    </nlm-citation>
  </ref>

  <ref id="B31">
    <label>31</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Nair</surname>
          <given-names>PN</given-names>
        </name>
        <name>
          <surname>Sj&#x00F6;gren</surname>
          <given-names>U</given-names>
        </name>
        <name>
          <surname>Krey</surname>
          <given-names>G</given-names>
        </name>
        <name>
          <surname>Kahnberg</surname>
          <given-names>KE</given-names>
        </name>
        <name>
          <surname>Sundqvist</surname>
          <given-names>E</given-names>
        </name>
      </person-group>
      <article-title>Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions : a long-term light and electron microscopic follow-up study</article-title>
      <source>J Endod</source>
      <year>1990</year>
      <volume>16</volume>
      <fpage>580</fpage>
      <lpage>588</lpage>
    </nlm-citation>
  </ref>

  <ref id="B32">
    <label>32</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Kerekes</surname>
          <given-names>K</given-names>
        </name>
        <name>
          <surname>Tronstad</surname>
          <given-names>L</given-names>
        </name>
      </person-group>
      <article-title>Morphometric observations on root canals of human anterior teeth</article-title>
      <source>J Endod</source>
      <year>1977</year>
      <volume>3</volume>
      <fpage>24</fpage>
      <lpage>29</lpage>
    </nlm-citation>
  </ref>

  <ref id="B33">
    <label>33</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Alani</surname>
          <given-names>AH</given-names>
        </name>
        <name>
          <surname>Toh</surname>
          <given-names>CG</given-names>
        </name>
      </person-group>
      <article-title>Detection of microleakage aroud dental restorations : a review</article-title>
      <source>Oper Dent</source>
      <year>1997</year>
      <volume>22</volume>
      <fpage>173</fpage>
      <lpage>185</lpage>
    </nlm-citation>
  </ref>

  <ref id="B34">
    <label>34</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Michailesco</surname>
          <given-names>PM</given-names>
        </name>
        <name>
          <surname>Valcarcel</surname>
          <given-names>J</given-names>
        </name>
        <name>
          <surname>Grieve</surname>
          <given-names>AR</given-names>
        </name>
        <name>
          <surname>Levallois</surname>
          <given-names>B</given-names>
        </name>
        <name>
          <surname>Lerner</surname>
          <given-names>D</given-names>
        </name>
      </person-group>
      <article-title>Bacterial leakage in endodontics</article-title>
      <source>J Endod</source>
      <year>1996</year>
      <volume>22</volume>
      <fpage>535</fpage>
      <lpage>539</lpage>
    </nlm-citation>
  </ref>

  <ref id="B35">
    <label>35</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Chaisrisookumporn</surname>
          <given-names>S</given-names>
        </name>
        <name>
          <surname>Rabinowitz</surname>
          <given-names>JL</given-names>
        </name>
      </person-group>
      <article-title>Evaluation of ionic leakage of lateral condensation and McSpadden methods by autoradiopraphy</article-title>
      <source>J Endod</source>
      <year>1982</year>
      <volume>8</volume>
      <fpage>493</fpage>
      <lpage>496</lpage>
    </nlm-citation>
  </ref>

  <ref id="B36">
    <label>36</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Czonstkowsky</surname>
          <given-names>M</given-names>
        </name>
        <name>
          <surname>Michanowicz</surname>
          <given-names>A</given-names>
        </name>
        <name>
          <surname>Vazquez</surname>
          <given-names>JA</given-names>
        </name>
      </person-group>
      <article-title>Evaluation of an injection of thermoplasticized low-temperature gutta-percha using radioactive isotopes</article-title>
      <source>J Endod</source>
      <year>1985</year>
      <volume>11</volume>
      <fpage>71</fpage>
      <lpage>74</lpage>
    </nlm-citation>
  </ref>

  <ref id="B37">
    <label>37</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Ravanshad</surname>
          <given-names>S</given-names>
        </name>
        <name>
          <surname>Torabinejad</surname>
          <given-names>M</given-names>
        </name>
      </person-group>
      <article-title>Coronal dye penetration of the apical filling materials after post space preparation</article-title>
      <source>Oral Surg Oral Med Oral Pathol</source>
      <year>1992</year>
      <volume>74</volume>
      <fpage>644</fpage>
      <lpage>647</lpage>
    </nlm-citation>
  </ref>

  <ref id="B38">
    <label>38</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Kos</surname>
          <given-names>WL</given-names>
        </name>
        <name>
          <surname>Aulozzi</surname>
          <given-names>DP</given-names>
        </name>
        <name>
          <surname>Gerstein</surname>
          <given-names>H</given-names>
        </name>
      </person-group>
      <article-title>A comparative bacterial microleakage study of retrofilling materials</article-title>
      <source>J Endod</source>
      <year>1982</year>
      <volume>8</volume>
      <fpage>355</fpage>
      <lpage>358</lpage>
    </nlm-citation>
  </ref>

  <ref id="B39">
    <label>39</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Kersten</surname>
          <given-names>HW</given-names>
        </name>
        <name>
          <surname>Moorer</surname>
          <given-names>WR</given-names>
        </name>
      </person-group>
      <article-title>Particles and molecules in endodontic leakage</article-title>
      <source>Int Endod J</source>
      <year>1989</year>
      <volume>22</volume>
      <fpage>118</fpage>
      <lpage>124</lpage>
    </nlm-citation>
  </ref>

  <ref id="B40">
    <label>40</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Usman</surname>
          <given-names>N</given-names>
        </name>
        <name>
          <surname>Baumgartner</surname>
          <given-names>JC</given-names>
        </name>
        <name>
          <surname>Marshall</surname>
          <given-names>JG</given-names>
        </name>
      </person-group>
      <article-title>Influence of instrument size on root canal debridement</article-title>
      <source>J Endod</source>
      <year>2004</year>
      <volume>30</volume>
      <fpage>110</fpage>
      <lpage>112</lpage>
    </nlm-citation>
  </ref>

  <ref id="B41">
    <label>41</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Coldero</surname>
          <given-names>LG</given-names>
        </name>
        <name>
          <surname>McHugh</surname>
          <given-names>S</given-names>
        </name>
        <name>
          <surname>MacKenzie</surname>
          <given-names>D</given-names>
        </name>
        <name>
          <surname>Saunders</surname>
          <given-names>WP</given-names>
        </name>
      </person-group>
      <article-title>Reduction in intracanal bacteria during root canal preparation with and without apical enlargement</article-title>
      <source>Int Endod J</source>
      <year>2002</year>
      <volume>35</volume>
      <fpage>437</fpage>
      <lpage>446</lpage>
    </nlm-citation>
  </ref>

  <ref id="B42">
    <label>42</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Green</surname>
          <given-names>D</given-names>
        </name>
      </person-group>
      <article-title>Stereomicroscopic study of 700 root apices of maxillary and mandibular posterior teeth</article-title>
      <source>Oral Surg Oral Med Oral Pathol</source>
      <year>1960</year>
      <volume>13</volume>
      <fpage>728</fpage>
      <lpage>733</lpage>
    </nlm-citation>
  </ref>

  <ref id="B43">
    <label>43</label>
        <nlm-citation citation-type="thesis">
          <person-group person-group-type="author">
            <name>
              <surname>West</surname>
              <given-names>JD</given-names>
            </name>
          </person-group>
          <source>The relation between the three-dimensional endodontic seal and endodontic failure</source>
          <year>1975</year>
          <publisher-loc>Boston</publisher-loc>
          <publisher-name>Boston University</publisher-name>
          <comment>master's thesis</comment>
        </nlm-citation>
      </ref>

  <ref id="B44">
    <label>44</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Barbakow</surname>
          <given-names>F</given-names>
        </name>
      </person-group>
      <article-title>The lightspeed system</article-title>
      <source>Dent Clin North Am</source>
      <year>2004</year>
      <volume>48</volume>
      <fpage>113</fpage>
      <lpage>135</lpage>
    </nlm-citation>
  </ref>

  <ref id="B45">
    <label>45</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Sedgley</surname>
          <given-names>CM</given-names>
        </name>
        <name>
          <surname>Messer</surname>
          <given-names>HH</given-names>
        </name>
      </person-group>
      <article-title>Are endodontically treated teeth more brittle?</article-title>
      <source>J Endod</source>
      <year>1992</year>
      <volume>18</volume>
      <fpage>332</fpage>
      <lpage>335</lpage>
    </nlm-citation>
  </ref>

  <ref id="B46">
    <label>46</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Stern</surname>
          <given-names>N</given-names>
        </name>
        <name>
          <surname>Hirshfeld</surname>
          <given-names>Z</given-names>
        </name>
      </person-group>
      <article-title>Principles of preparing endodontically treated teeth for dowel and core restorations</article-title>
      <source>J Prosthet Dent</source>
      <year>1973</year>
      <volume>30</volume>
      <fpage>162</fpage>
      <lpage>165</lpage>
    </nlm-citation>
  </ref>

  <ref id="B47">
    <label>47</label>
    <nlm-citation citation-type="journal">
      <person-group person-group-type="author">
        <name>
          <surname>Tilk</surname>
          <given-names>MA</given-names>
        </name>
        <name>
          <surname>Lommel</surname>
          <given-names>TJ</given-names>
        </name>
        <name>
          <surname>Gerstein</surname>
          <given-names>H</given-names>
        </name>
      </person-group>
      <article-title>A study of mandibular and maxillary root widths to determine dowel size</article-title>
      <source>J Endod</source>
      <year>1979</year>
      <volume>5</volume>
      <fpage>79</fpage>
      <lpage>82</lpage>
    </nlm-citation>
  </ref>

    </ref-list>

</back>

<floats-wrap>

<fig position="float" id="F1">
<label>Figure 1</label>
<caption>
  <p>Positive control (left), Negative control (right).</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="jkacd-33-66-g001" alt-version="no"></graphic>
</fig>

<fig position="float" id="F2">
<label>Figure 2</label>
<caption>
  <p>Comparison of dye leakage (&#x00D7; 20).</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="jkacd-33-66-g002" alt-version="no"></graphic>
</fig>

<fig position="float" id="F3">
<label>Figure 3</label>
<caption>
  <p>Stereomicroscopic view of cross-section at 1, 2, 3, 4 mm from the apex (&#x00D7; 83).</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="jkacd-33-66-g003" alt-version="no"></graphic>
</fig>

<table-wrap position="float" id="T1">
<label>Table 1</label>
<caption>
  <p>Minimum, maximum, mean and standard deviation (S.D) value of apical microleakage (mm)</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="jkacd-33-66-i001" alt-version="no"></graphic>
</table-wrap>

</floats-wrap>

</article>