Korean J Nutr KJN Korean Journal of Nutrition 0367-6463 2005-7121 The Korean Nutrition Society 10.4163/kjn.2011.44.5.378 Research Article The Effect of Supplementary Selenium on Leukocytes and HSP70 Expression after Half-Body Immersion Shin Young-Oh 1 Han Min-Kyu 1 Lee Jeong-Beom 2 Um Byung-Hun 3 Department of Health Care, Graduate School, Soonchunhyang University, Asan 336-745, Korea. Department of Physiology, College of Medicine, Soonchunhyang University, Chonan 331-946, Korea. Functional Food Center, KIST Gangneung 210-340, Korea. To whom correspondence should be addressed. (leejb@sch.ac.kr) 10 2011 31 10 2011 44 5 378 383 12 07 2011 09 08 2011 16 09 2011 © 2011 The Korean Nutrition Society 2011

This study examined the effect of supplementary selenium on leukocytes and heat shock protein (HSP) 70 expression in serum during half-body immersion. The subjects were male college tennis athletes. All subjects participated in two repeated experiments with a 1 week interval. During the 30 min intermittent half-body immersion, subjects were given 500 mL of water with or without selenium (100 µg). Blood samples were taken from the antecubital vein, and differential counts were made. Serum HSP70 protein was analyzed using a commercial ELISA kit. After half-body immersion, leukocytes and lymphocytes increased significantly but neutrophils decreased significantly in both trials (with or without selenium). Selenium supplementation, compared with placebo, decreased levels of leukocytes, neutrophils, and monocytes, but not lymphocytes, to the resting level or below 60 min after immersion. Only lymphocytes continued to increase in both trials during the recovery period. Serum HSP70 protein level did not change after immersion, but it decreased 60 min after immersion with the administration of selenium. In conclusion, supplementary selenium reduced the systemic immune response and serum HSP70 protein accumulation after half-body immersion.

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Changes of leukocytes and subsets over time in two treatments. White bar: water and placebo, black bar: water and selenium.

*: p < 0.05, **: p <0.01, ***: p <0.001; compare with Pre in each treatment.

HSP70 expression over time in two treatments. White bar: water and placebo, black bar: water and selenium. *: p <0.05. compare with Pre in each treatment.

Characteristics of subjects

Mean ± SD

Changes of leukocytes and HSP70 protein over time in two treatments

Mean ± SD

*: p < 0.05, **: p<0.01, ***: p < 0.001; compare with Pre in each treatment