jkap The Journal of Korean Academy of Prosthodontics J Korean Acad Prosthodont 0301-2875 2005-3789 The Korean Academy of Prosthodontics 10.4047/jkap.2013.51.2.82 jkap-51-82 ORIGINAL ARTICLE Combined effects of rhBMP-2 and rhVEGF coated onto implants on osseointegration: pilot study HuhJung-BoMSD, DDS, PhD 1 YunMi-JungMSD, DDS, PhD 1 JeongChang-MoMSD, DDS, PhD 1 ShinSang-WanMSD, DDS, PhD 2 JeonYoung-ChanDDS, MSD, PhD 1 ∗ Department of Prosthodontics, School of Dentistry, Pusan National University, Yangsan, Korea Department of Prosthodontics, Institute for Clinical Dental Research, Korea University Guro Hospital, Seoul, Korea ∗Corresponding Author: Young-Chan Jeon Department of Prosthodontics, School of Dentistry, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan, 626-870, Korea +82 55 360 5130: e-mail, jeonyc@paran.com 12 2013 18 12 2013 51 2 82 89 08032013 03042013 04042013 Copyright © 2013 The Korean Academy of Prosthodontics 2013 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Purpose

The present study is aimed to evaluate the combined effect of recombinant human bone morphogenetic protein 2 (rhBMP-2) and recombinant human vascular endothelial growth factor (rhVEGF) coated onto anodized implants on osseointeration.

 Materials and methods

Six New Zealand white rabbit were used in this study. Each animal received 4 implants that were either coated with rhBMP-2 and rhVEGF (Study group) or anodized implant (Control group) in both tibia. This was performed using a randomized split-mouth design. A total 24 implants were used. The implant stability quotient (ISQ) value using resonance frequency analyser and removal torque (RTQ) measurement were investigated at 2 and 8 weeks. The t-test was used for statistical analysis (α =.05).

 Results

Control and study group showed good osseointegration at 8 weeks. The ISQ and RTQ values of study group were significant compared with the control group at 8 weeks (P<.05). However, No statistical significance was observed at 2 weeks (P>.05).

 Conclusion

It was concluded that rhBMP-2 with rhVEGF coated onto anodized implants can induce better osseointegration at late healing period. (J Korean Acad Prosthodont 2013;51:82-9)

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Procedures of surgery in rabbit tibia. A: marking the incision area, B: exposure of tibia, C: marking the insertion portion, D: implant insertion.

The measurements of implant stability and removal torque. A: Osstell Mentor for implant stability quotient value, B: removal torque gauge MGT12 for removal torque measurement.

Mean and standard deviation of ISQ values in each group.

Mean and standard deviation of RTQ values in each group.

The means and SD of each group in different intervals

Group ISQ value (mean ± SD) RTQ value (mean ± SD)
Post OP 2 weeks 8 weeks 2 weeks 8 weeks
Control 55.67 ± 13.94 60.50 ± 6.83 66.83 ± 3.66 20.99 ± 3.48 30.28 ± 6.50
Study 56.34 ± 16.77 54.33 ± 10.02 77.83 ± 6.18 20.61 ± 3.59 43.42 ± 7.93

RTQ: removal torque measurements, ISQ: implant stability quotient.