kjh The Korean Journal of Hematology Korean J Hematol 1738-7949 2092-9129 Korean Society of Hematology 10.5045/kjh.2009.44.1.36 kjh-44-36 Original Article Effect of Platelet Transfusion on the Platelet Parameters of Recipient LimYoung Ae ChoSung Ran Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea Correspondence to：Young Ae Lim, M.D. Department of Laboratory Medicine, Ajou University School of Medicine San 5, Woncheon-dong, Yeongtong-gu, Suwon 443-721, Korea Tel: ＋82-31-219-5786, Fax: ＋82-31-219-5778 E-mail: limyoung@ajou.ac.kr 032009 20032009 441 3641 26122008 10022009 15022009 Copyright © 2009 Korean Society of Hematology 2009 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background

Because the platelet parameters have recently been used as indicators for various clinical conditions, there is a need to accurately estimate the platelet parameters by using an automated hematology analyzer. The aim of this study was to investigate an effect of a platelet transfusion on such platelet parameters as the mean platelet volume (MPV), the platelet volume distribution width (PDW), the mean platelet component (MPC) and the platelet component distribution width (PCDW) in transfused patients.

 Methods

The study subjects were 25 patients who were admitted to the Department of Hematology & Oncology and they had been transfused with platelets. CD62P labeling was performed in the remaining portions of single donor platelets products (SDP) and the pooled platelet concentrates (PC) just before the SDP and PC were released. The platelet parameters were determined using the ADVIA 120 and the whole blood samples from the patients before the platelet transfusions and within 10∼16 hours after the transfusion.

 Results

There were no significant difference of all the platelet parameters between the SDP (n=21) and the pooled PC (n=8). The MPC and PCDW of the SDP and the PC were significantly lower than those of the samples from the patients before transfusion. However, the PCDW of the samples from the patients after transfusion was significantly lower than that before transfusion.

 Conclusion

Because platelet transfusions lower the value of the PCDW in patients, the laboratory staff and clinicians should be aware of this when interpreting the PCDW.

 Platelet transfusion Platelet parameter Platelet component distribution width References Kim HK Kim JE Ham CK Lee DS Park S Cho HI Prognostic value of platelet indices as determined by ADVIA 120 in patients suspected of having disseminated intravascular coagulation Int J Lab Hematol 200830 11723 Zuberi BF Akhtar N Afsar S Comparison of mean platelet volume in patients with diabetes mellitus, impaired fasting glucose and non-diabetic subjects Singapore Med J 200849 1146 Coban E Adanir H Bilgin D The association of mean platelet volume levels with hypertensive retinopathy Platelets 200819 1158 Ntaios G Papadopoulos A Chatzinikolaou A Increased values of mean platelet volume and platelet size deviation width may provide a safe positive diagnosis of idiopathic thrombocytopenic purpura Acta Haematol 2008119 1737 Ihara A Matsumoto K Kawamoto T Evaluation of platelet indexes in patients with aortic aneurysm Pathophysiol Haemost Thromb 200534 26973 Macey MG Carty E Webb L Use of mean platelet component to measure platelet activation on the ADVIA 120 haematology system Cytometry 199938 2505 Lim YA Hyun BH Evaluation of platelet parameters on the ADVIA 120 as the quality indicator for stored platelets Clin Lab Haematol 200224 37784 Boos CJ Beevers GD Lip GY Assessment of platelet activation indices using the ADVIATM 120 amongst ‘high-risk' patients with hypertension Ann Med 200739 728 Dastjerdi MS Emami T Najafian A Amini M Mean platelet volume measurement, EDTA or citrate? Hematology 200611 3179 Lim YA Cho SR Lee WG Park JS Kim SW Change of platelet activation markers using flow cytometry in patients with hematology/oncology disorders after transfusion Platelets 200819 32834 Brecher ME, ed Technical manual 15th ed. Bethesda, USA American Association of Blood Banks 2005 363 Ness PM Campbell-Lee SA Single donor versus pooled random donor platelet concentrates Curr Opin Hematol 20018 3926 de Vries RA de Bruin M Marx JJ Hart HC Van de Wiel A Viability of platelets collected by apheresis versus the platelet-rich plasma technique: a direct comparison Transfus Sci 199314 3918 Turner VS Hawker RJ Mitchell SG Seymour Mead AM Paired in vivo and in vitro comparison of apheresis and "recovered" platelet concentrates stored for 5 days J Clin Apher 19949 18994 Arnold DM Heddle NM Kulczycky M Carruthers J Sigouin C Blajchman MA In vivo recovery and survival of apheresis and whole blood-derived platle-lets: a paired comparison in healthy volunteers Transfusion 200646 25764 Van der Lelie J Von dem Borne AK Platelet volume analysis for differential diagnosis of thrombocytosis J Clin Pathol 198639 12933 Michelson AD Barnard MR Hechtman HB In vivo tracking of platelets: circulating degranulated platelets rapidly lose surface P-selectin but continue to circulate and function Proc Natl Acad Sci USA 199693 1187782 Ruf A Patscheke H Flow cytometric detection of activated platelets: comparison of determining shape change, fibrinogen binding, and P-selectin expression Semin Thromb Hemost 199521 14651 Berger G Hartwell DW Wagner DD P-Selectin and platelet clearance Blood 199892 444652 Reed GL Platelet secretion Michelson AD Platelets 1st ed. London, England Academic Press 2006 18195 Figures and Tables

The correlation between platelet count in patients after transfusion and mean platelet volume (MPV) in patients before transfusion (A) or the expression of CD62P in platelet products (B).

Comparison of platelet parameters on the ADVIA 120, storage time, and CD62P expression (%) in pooled platelet concentrates (PC) and single donor platelet pheresis products (SDP); and corrected count of increments in patients transfused

  PC (n=8) SDP (n=21)
MPV (fL) 8.96±0.66 8.55±0.81
PDW (%) 49.93±2.97 52.20±3.32
MPC (g/dL) 20.66±0.75 20.80±1.73
PCDW (g/dL) 6.38±0.60 6.29±1.10
CD62P (%) 24.6±5.8 27.1±18.2
Storage time (hours) 48.5±33.9 38.1±18.4
CCI in patients transfused 6,356±5,725 6,838±4,367

MPV, mean platelet volume; PDW, platelet volume distribution width; MPC, mean platelet component; PCDW, platelet component distribution width. One unit of pooled PC originated from 8 units of PC. There was no significant difference of platelet parameters on the ADVIA 120, CD62P expression and storage time in products or CCI in patients transfused between PC and SDP.

The change of platelet parameters, on the ADVIA 120, in patients transfused and platelet parameters of pooled platelet concentrates (PC) and single donor platelet products (SDP)

  PC (n=8) Patients transfused with PC SDP (n=21) Patients transfused with SDP
Before (n=8) After (n=8) Before (n=21) After (n=21)
PLT∗ 3.54±0.94 18,125±8,236 31,875±11,969‡ 3.50±0.42 12,571±4,319 27,380±12,978‡
MPV (fL) 8.96±0.66 8.46±0.91 8.53±0.65 8.55±0.81 8.40±0.91 8.58±0.94
PDW (%) 49.93±2.97† 57.93±9.25 51.84±8.60† 52.20±3.32 53.36±14.16 52.46±11.02
MPC (g/dL) 20.66±0.75‡ 23.54±1.48 23.66±1.72 20.80±1.73‡ 23.38±1.98 24.10±2.09
PCDW (g/dL) 6.38±0.60† 7.09±0.74 6.54±0.43† 6.29±1.10‡ 7.29±0.75 6.73±0.76†

Abbreviation: See Table 1. ∗×1011 for platetlet products and ×10

/L for patients transfused;

P＜0.05 versus before platelets transfusion; versus before platelets transfusion.

P＜0.01