kjpp The Korean Journal of Physiology & Pharmacology Korean J Physiol Pharmacol 1226-4512 2093-3827 Korean J Physiol Pharmacol 10.4196/kjpp.2011.15.5.307 kjpp-15-307 Original Article Roles of Non-cholinergic Intrapancreatic Nerves, Serotonergic Nerves, on Pancreatic Exocrine Secretion in the Isolated Perfused Rat Pancreas JiangZhenger 1 ShinBich-Na 2 KimIn-Hye 2 LeeHyun Joo 2 YongJun-Hwan 3 LeeMin-Jae 4 WonMoo-Ho 5 LeeYun-Lyul 2 Department of Physiology, College of Nursing, Yanbian University, Yanji 133000, China Department of Physiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea Department of Occupational Therapy, Dongnam Health College, Suwon 440-714, Korea Department of Veterinary Lab. Animal Medicine & Science, College of Animal Resource Science, Kangwon National University, Chuncheon 200-701, Korea Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, Korea Corresponding to: Yun-Lyul Lee, Department of Physiology, College of Medicine, Hallym University, 39, Hallymdaehak-gil, Chuncheon 200-702, Korea. (Tel) 82-33-248-2583, (Fax) 82-33-248-1640, (E-mail) yylee@hallym.ac.kr 022011 18022011 15 5 307 312 21092011 14102011 17102011 Copyright © 2011 Korean J Physiol Pharmacol 2011 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract

It has been rereported that axons which display 5-hydroxytryptamine (5-HT) immunoreactivity are abundant in the pancreas and the majority of serotonergic axons terminate within intrapancreatic ganglia, islet and acini. This histological result strongly suggests that intrapancreatic serotonergic nerves could affect to the pancreatic endocrine and exocrine secretion. Thus, this study was aimed to investigate whether intrapancreatic serotonergic nerves could affect pancreatic exocrine secretion and an action mechanism of the intrapancreatic serotonergic nerves. The rats were anesthetized with a single injection of urethane. The median line and the abdominal aorta was carefully dissected and cannulated with PE-50 tubing just above the celiac artery, and then tightly ligated just below the superior mesenteric artery. The pancreatic duct was also cannulated with Tygon microbore tubing. With the addition of serotonin, pancreatic volume flow and amylase output were significantly inhibited electrical field stimulation (EFS). On the other hand, pancreatic volume flow and amylase output were significantly elevated in EFS with the addition of spiperone. EFS application, however, pancreatic volume flow and amylase output had no significant change in cholecystokinin (CCK) alone when serotonin was applied under a 5.6 mM glucose background. Pancreatic volume flow and amylase output under 18 mM glucose background were significantly elevated in CCK plus serotonin than in CCK alone. These data suggest that intrapancreatic serotonergic nerves play an inhibitory role in pancreatic exocrine secretion and an important role in the insulin action or release.

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Effect of serotonin (5-HT, 2μM) on electrical field stimulation (EFS, 12 V, 2 ms, 8 Hz)-induced pancreatic volume flow and amylase output. Each points (❍, EFS alone; , EFS + 5-HT) represent the mean±S.E. of 7 experiments. ∗The value is significantly (p<0.05) different from that of EFS alone.

Effect of spiperone hydrochloride (5-HT antagonist, 5μM) on electrical field stimulation (EFS, 12 V, 2 ms, 8 Hz)-induced pancreatic volume flow and amylase output. Each points (❍, EFS alone; , EFS + 5-HT antagonist) represent the mean±S.E. of 6∼7 experiments. ∗The value is significantly (p<0.05) different from that of EFS alone.

Effect of spiperone hydrochloride (5-HT antagonist, 5μM) with atropine (ATR, 2μM) on electrical field stimulation (EFS, 12 V, 2 ms, 8 Hz)-induced pancreatic volume flow and amylase output. Each points (❍, EFS + ATR; , EFS + ATR + 5-HT antagonist) represent the mean±S.E. of 6∼7 experiments. ∗The value is significantly (p<0.05) different from that of EFS plus atropine.

Effect of serotonin (5-HT, 2μM) on cholecystokinin (CCK, 5 pmol)-induced pancreatic volume flow and amylase output under 5.6 mM glucose background. Each points (❍, CCK alone; , CCK+ 5-HT) represent the mean±S.E. of 6 experiments. ∗The value is significantly (p<0.05) different from that of CCK alone.

Effect of serotonin (5-HT, 2μM) on cholecystokinin (CCK, 5 pmol)-induced pancreatic volume flow and amylase output under 18 mM glucose background. Each points (❍, CCK alone; , CCK+ 5-HT) represent the mean±S.E. of 6 experiments. ∗The value is significantly (p<0.05) different from that of CCK alone.