JKOS Journal of the Korean Ophthalmological Society J Korean Ophthalmol Soc 0378-6471 2092-9374 Korean Ophthalmological Society 10.3341/jkos.2017.58.1.69 jkos-58-69 Original Article Effect of Tetrahydrozoline on the Permeability of Trabecular Meshwork Cell Monolayer HwangSeok Jin M.D KimJae Woo MD, PhD Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center, #33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 42472, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133 E-mail: jwkim@cu.ac.kr 08 2017 30 8 2017 58 1 69 73 25 8 2016 18 10 2016 7 12 2016 Copyright © 2017 Korean Ophthalmological Society 2017 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Purpose

To investigate the effects of tetrahydrozoline (THZ) on the survival of cultured human trabecular meshwork cells (HTMC) and the permeability of HTMC monolayer.

 Methods

Primary cultured HTMC were exposed to an adrenergic agonist (0.01, 0.1, 1.0 or 10 μ M THZ) for 1 day and 3 days. Carboxyfluorescein permeability through the HTMC monolayer was measured using Transwell. Cellular viability and nitric oxide (NO) production were assessed using MTT and Griess assays, respectively.

 Results

THZ did not affect the cellular survival ( p > 0.05) or NO production ( p > 0.05). THZ significantly increased the carboxy-fluorescein permeability through the HTMC monolayer in a dose-dependent manner compared with non-exposed control ( p < 0.05) after exposure for 1 and 3 days.

 Conclusions

THZ does not affect the survival of HTMC but decreases the permeability of HTMC monolayer in a dose-depend-ent manner. Thus, THZ may possibly decrease trabecular outflow.

 Permeability Survival Tetrahydrozoline Trabecular meshwork cells References AlvaradoJMurphyCJusterRTrabecular meshwork cellularity in primary open-angle glaucoma and nonglaucomatous normalsOphthalmology19616611124 RohenJWLütjen-drecollEFlügelCUltrastructure of the trabecular meshwork in untreated cases of primary open-angle glaucomaExp Eye Res19616611124 AraieMCarboxyfluorescein. A dye for evaluating the corneal en-dothelial barrier function in vivoExp Eye Res19616611124 AraieMBarrier function of corneal endothelium and the intra-ocular irrigating solutionsArch Ophthalmol19616611124 TsuboiSPedersonJEPermeability of the isolated dog retinal pig-ment epithelium to carboxyfluoresceinInvest Ophthalmol Vis Sci19616611124 BlairNPRusinMMBlood-retinal barrier permeability to carbox-yfluorescein and fluorescein in monkeysGraefes Arch Clin Exp Ophthalmol19616611124 GrimesPACarboxyfluorescein transfer across the blood-retinal barrier evaluated by quantitative fluorescence microscopy: com-parison with fluoresceinExp Eye Res19616611124 NakagawaSUsuiTYokooSToxicity evaluation of anti-glaucoma drugs using stratified human cultivated corneal epi-thelial sheetsInvest Ophthalmol Vis Sci19616611124 KamedaTInoueTInataniMThe effect of Rho-associated protein kinase inhibitor on monkey Schlemm’s canal endothelial cellsInvest Ophthalmol Vis Sci19616611124 RaoPVDengPFKumarJEpsteinDLModulation of aqueous humor outflow facility by the Rho kinase–specific inhibitor Y-27632Invest Ophthalmol Vis Sci19616611124 MengerHCNew ophthalmic decongestant, tetrahydrozoline chloride; clinical use in 1,156 patients with conjunctival irritationJ AmMed Assoc19616611124 MosmannTRapid colorimetric assay for cellular growth and sur-vival: application to proliferation and cytotoxicity assaysJ Immunol Methods19616611124 FremoserFMJakobCAAebiMTuorUThe MTT[3-(4,5- dime-thylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densitiesAppl Environ Microbiol19616611124 Green,LCWagnerDAGlogowskiJAnalysis of nitrate, ni-trite and [15N]nitrate in biologic fluidsAnalytical Biochem19616611124 O'DonnellMEBrandtJDCurryFRNa-K-Cl cotransport regu-lates intracellular volume and monolayer permeability of tra-becular meshwork cellsAm J Physiol1995268(4 Pt 1)C106774 WiederholtMSchäferRWagnerULepple-WienhuesAContractile response of the isolated trabecular meshwork and cil-iary muscle to cholinergic and adrenergic agentsGer J Ophthalmol19616611124 ButlerKThompsonJPYoltonDPEffects of non-prescription oc-ular decongestantsRev Optom19616611124 StamerWDLeiYBoussommier-CallejaAeNOS, a pres-sure-dependent regulator of intraocular pressureInvest Ophthalmol Vis Sci19616611124 PredescuDPredescuSShimizuJConstitutive eNOS-de-rived nitric oxide is a determinant of endothelial junctional integrityAm J Physiol Lung Cell Mol Physiol2005289L37181 DismukeWMMbadughaCCEllisDZNO-induced regulation of human trabecular meshwork cell volume and aqueous humor out-flow facility involve the BKCa ion channelAm J Physiol Cell Physiol2008294C137886 KimHYKimJWEffect of nitric oxide on the permeability of tra-becular meshwork cell monolayerJ Korean Ophthalmol Soc19616611124 VidrioHPardoEGAntihypertensive effects of sympathomimetic aminesJ Pharmacol Exp Ther19616611124 RazeghinejadMRKatzLJSteroid-induced iatrogenic glaucomaOphthalmic Res19616611124 Figures

Effect of tetrahydrozoline on the survival of con-fluently cultured trabecular meshwork exposed for 1 day. Tertrahydrozoline did not affect on the survival significantly compared to non-exposed control ( p > 0.05).

Effect of tetrahydrozoline on the survival of con-fluently cultured trabecular meshwork exposed for 3 days. Tertrahydrozoline did not affect on the survival significantly compared to non-exposed control ( p > 0.05).

Effects of tetrahydrozoline on the permeability of car-boxyfluorescin through the trabecular meshwork cell monolayer exposed for 1 day. 1.0, 10.0 μ M tetrahydrozoline decreased the permeability of carboxyfluorescin significantly compared to non-exposed control (* p < 0.05). Carboxyfluorescein intensity of outer chamber normalized to the mean value obtained using non-exposed control (permeability 100%).

Effects of tetrahydrozoline on the permeability of car-boxyfluorescin through the trabecular meshwork cell monolayer exposed for 3 days. 0.1, 1.0, and 10.0 μ M tetrahydrozoline de-creased the permeability of carboxyfluorescin significantly com-pared to non-exposed control (* p < 0.05). Carboxyfluorescein intensity of outer chamber normalized to the mean value obtained using non-exposed control (permeability 100%).