Tuberc Respir Dis TRD Tuberculosis and Respiratory Diseases 1738-3536 2005-6184 The Korean Academy of Tuberculosis and Respiratory Diseases 10.4046/trd.2007.62.2.105 Original Article The Efficacy of α-lipoic Acid on the Endotoxin-induced Acute Lung Injury Huh Jin Won M.D. 1 Hong Sang Bum M.D. 2 Kim Mi Jung M.D. 3 Lim Chae-Man M.D. 2 Koh Younsuck M.D. 2 Department of Internal Medicine, Ilsan Paik Hospital, Inje University, Goyang, Korea. Division of Pulmonary and Critical Care Medicine, Asan Medical Center, University of ulsan College of Medicine, Seoul, Korea. Asan Institute for Life Sciences, Seoul, Korea. Address for correspondence: Younsuck Koh, M.D. Division fo Pulmonary & Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap Dong, Songpa Ku, Seoul, 138-736, Korea. Fax: 02-3010-3134, Phone: 02-3010-6968, yskoh@amc.seoul.kr 02 2007 28 02 2007 62 2 105 112 11 12 2006 09 02 2007 Copyright © 2007 The Korean Academy of Tuberculosis and Respiratory Diseases 2007 Background

Oxidative stress may play an important role in the pathogenesis of endotoxin-induced acute lung injury (ALI). This study evaluated the therapeutic effect of α-lipoic acid, a nonenzymatic antioxidant, in a rat model of lipopolysaccharide (LPS) induced ALI.

 Materials and Methods

ALI was induced in Sprague-Dawley rats by instilling LPS (E.coli, 3mg/Kg) into the trachea. The rats were classified into the control, control+α-lipoic acid, LPS, and LPS+α-lipoic acid groups.The lung lavage neutrophil count, cytokine-induced neutrophil chemoattractant (CINC), lung myeloperoxidase (MPO), and cytokine concentrations (TNF-α, IL-1β, IL-6 and IL-10) were measured at 2 h and 6 h after LPS administration.

 Results

The total cell and neutrophil counts of the LPS+α-lipoic acid groups were significantly lower than the LPS groups. The protein concentration in the BAL fluid was similar in the LPS groups and LPS+α-lipoic acid groups. The TNF-α, IL-1β, and IL-6 concentrations in the BAL fluid were not decreased by the α-lipoic acid treatment in the LPS treated rats.

 Conclusions

Although α-lipoic acid decreased the level of LPS-induced neutrophil infiltration into the lung, it could not attenuate the LPS-induced ALI at the dose administered in this study.

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The effect of α-lipoic acid on the level of CINC of BAL fluid in rats treated with LPS injection (L: LPS group, LA: LPS+α-lipoic acid group) (*p<0.05)

The effect of α-lipoic acid on the level of MIP of BAL fluid in rats treated with LPS injection (L: LPS, LA: LPS+α-lipoic acid) (*p<0.05)

The effect of α-lipoic acid on the level of cytokines of BAL fluid in rats treated with LPS (C: control, A: α-lipoic, L: LPS, LA: LPS+α-lipoic acid) (*p<0.05)

Total cell count and neutrophil count in BAL fluid at 2 hour after LPS intratracheal (IT) injection

*p value; versus Control group, †p value; versus LPS group

Total cell count and neutrophil count in BAL fluid at 6 hour after LPS injection

*p value; versus Control group, †p value; versus LPS group

Lung myeloperoxidase (MPO) after LPS injection

*p value; versus Control group, †p value; versus LPS group

Protein concentration in BAL fluid after LPS injection

*p value; versus Control group, †p value; versus LPS group