Evaluation of the Diagnostic Performance of the AdvanSure TB/NTM Real-Time PCR Kit for Detection of Mycobacteria

Hwang, Sangsun; Oh, Ki Jin; Jang, In Ho; Uh, Young; Yoon, Kap Jun; Kim, Hyo Youl; Kim, Young Keun

doi:10.5145/KJCM.2011.14.2.55

Original Article


Korean J Clin Microbiol. 2011 Jun;14(2):55-59. Korean. Published online June 29, 2011. https://doi.org/10.5145/KJCM.2011.14.2.55
Copyright © 2011 The Korean Society of Clinical Microbiology


Evaluation of the Diagnostic Performance of the AdvanSure TB/NTM Real-Time PCR Kit for Detection of Mycobacteria
Sangsun Hwang,¹ Ki Jin Oh,¹ In Ho Jang,¹ Young Uh,¹ Kap Jun Yoon,¹ Hyo Youl Kim,² and Young Keun Kim²
¹Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
²Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
Correspondence: Young Uh, Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, 162 Ilsan-dong, Wonju 220-701, Korea. (Tel) 82-33-741-1592, (Fax) 82-33-731-0506, Email: u931018@yonsei.ac.kr

Received July 06, 2010; Revised November 30, 2010; Accepted March 05, 2011.
This article has been cited by 9 articles in This article has been cited by 6 articles in KoMCI. This article has been cited by Google Scholar.
Go to: Abstract
Background The AdvanSure TB/NTM real-time PCR kit (AdvanSure) was newly developed in Korea to detect Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) utilizing a specific primer and TaqMan probe targeting the IS6110 and rpoB genes which are unique to these species. The purpose of the present study was to evaluate the clinical utility of AdvanSure by comparing the results of acid-fast staining, mycobacteria culture, COBAS Amplicor MTB PCR (Amplicor), and AdvanSure. Methods A total of 182 specimens (105 respiratory and 77 nonrespiratory specimens) were obtained from 165 patients, and acid fast bacilli (AFB) staining, mycobacteria culture, and Amplicor were performed on all specimens. AdvanSure was also performed on the above specimens using the SLAN real-time PCR detection system. The sensitivity and specificity of AdvanSure were analyzed using AFB staining and culture. Results Of the 182 specimens, M. tuberculosis was detected in 43 specimens and NTM was detected in 12 specimens according to PCR and/or culture. The sensitivity and specificity of the AdvanSure based on AFB culture were 97.3% (36/37) and 95.5% (127/133) in M. tuberculosis and 75.0% (9/12) and 100% (0/133) in NTM, respectively. Conclusion AdvanSure could be useful for detecting M. tuberculosis and NTM in the clinical laboratory with high sensitivity and specificity.


Keywords: Mycobacterium tuberculosis; Nontuberculous mycobacteria; Real-time PCR

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Tables


	Table 1 Detection frequency of mycobacteria by based on PCR and culture results
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	Table 2 Sensitivity and specificity of acid-fast bacilli (AFB) stain and culture, and two PCR methods for detection of mycobacteria based on combination of PCR and culture results
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	Table 3 Comparison of two PCR results according to AFB stain and culture
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	Table 4 Sensitivity of two PCR methods for M. tuberculosis according to type of specimens based on combination of PCR and culture methods
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