Journal List > Korean J Nutr > v.43(4) > 1043831

Baik, Ahn, Lee, Kim, Han, and Shin: Genetic Variants Associated with Calorie and Macronutrient Intake in a Genome-Wide Association Study

Abstract

There has been no genome-wide association study (GWAS) for macronutrient intake as a quantitative trait. To explore genetic loci associated with total calorie and macronutrient intake, genome-wide association data of autosomal single nucleotide polymorphisms (SNPs) from Korean adults were analyzed. We conducted a GWAS in 3,690 men and women aged 40 to 60 years from an urban population-based cohort. At the baseline examination (June 18, 2001 through January 29, 2003), DNA samples of the study subjects were collected and analyzed for genotyping. The information of average daily consumption of total calorie, carbohydrate, protein, and fat was obtained from a semi-quantitative food frequency questionnaire and transformed by natural logarithm for analyses after adjustment of calorie intake. Using multivariate linear regression analysis adjusted for age, sex, and height, we tested for 352,021 SNPs and found weak associations, which do not reach genome-wide association significance, with calorie and macronutrient intake. However, a number of SNPs were found to have potential associations with macronutrient intake; in particular, signals in SORBS1 and those in PRKCB1 were likely associated with carbohydrate and fat intake, respectively. We observed an inverse association between the minor allele of the SNPs in these genes and the amount of consumption of carbohydrate or fat. Our GWAS identified loci and minor alleles weakly associated with macronutrient intake. Because SORBS1 and PRKCB1 are reportedly associated with the metabolism of glucose and lipid as well as with obesity-related diseases, further investigations on biological and functional roles of polymorphism of these genes in the relation to macronutrient intake are warranted.

Figures and Tables

Fig. 1
Quantile-quantile (QQ) plots of the -log10 p-values from the additive model-based analysis of genome-wide association data for average daily intake of total calorie and macronutrients among 3,690 study subjects. Amounts of carbohydrate, protein, and fat consumption have been adjusted for total calorie and log-transformed.
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Fig. 2
Association analysis of SNPs in SORBS1 gene region on chromosome 10q23.33. The panel shows p-values for the association testing of genome-wide association data with natural log transformed calorie-adjusted carbohydrate intake. The association was drawn from linear regression analysis adjusted for sex, age, and height on the basis of additive model. The Y axis is the negative log10 p-values and the × axis is position of chromosome 10q23.
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Fig. 3
Association analysis of SNPs in PRKCB1 gene region on chromosome 16p12.1. The panel shows p-values for the association testing of genome-wide association data with natural log transformed calorie-adjusted fat intake. The association was drawn from linear regression analysis adjusted for sex, age, and height on the basis of dominant model. The Y axis is the negative log10 p-values and the × axis is position of chromosome 16p12.
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Table 1
Characteristics of the study population (n = 3,690)
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SD: standard deviation

Table 2
SNPs showing the smallest unadjusted p-values (< 10-5) for the association with natural log transformed calorie intake according to additive, dominant, and recessive models
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SNP: single-nucleotide polymorphism, M/m alleles: major and minor alleles, MAF: minor allele frequency, Beta: the effect size on total calorie intake, SE: standard error

1) Unadjusted p-value was obtained from multivariate models treating sex, age, and height as covariates

2) Frequency of other SNPs in the same gene showing p < 0.001

Table 3
SNPs showing the smallest unadjusted p-values (< 10-5) for the association with natural log transformed calorie-adjusted carbohydrate intake according to additive, dominant, and recessive models
kjn-43-357-i003

SNP: single-nucleotide polymorphism, M/m alleles: major and minor alleles, MAF: minor allele frequency, Beta: the effect size on total calorie intake, SE: standard error

1) Unadjusted p-value was obtained from multivariate models treating sex, age, and height as covariates

2) Frequency of other SNPs in the same gene showing p < 0.001

Table 4
SNPs showing the smallest unadjusted p-values (< 10-5) for the association with natural log transformed calorie-adjusted protein intake according to additive, dominant, and recessive models
kjn-43-357-i004

SNP: single-nucleotide polymorphism, M/m alleles: major and minor alleles, MAF: minor allele frequency, Beta: the effect size on total calorie intake, SE: standard error

1) Unadjusted p-value was obtained from multivariate models treating sex, age, and height as covariates

2) Frequency of other SNPs in the same gene showing p < 0.001

Table 5
SNPs showing the smallest unadjusted p-values (< 10-5) for the association with natural log transformed calorie-adjusted fat intake according to additive, dominant, and recessive models
kjn-43-357-i005

SNP: single-nucleotide polymorphism, M/m alleles: major and minor alleles, MAF: minor allele frequency, Beta: the effect size on calorie-adjusted fat intake, SE: standard error

1) Unadjusted p-value was obtained from multivariate models treating sex, age, and height as covariates

2) Frequency of other SNPs in the same gene showing p < 0.001

Notes

This study was supported by a grant of the Korea Centers for Disease Control and Prevention (budgets 2001-347-6111-221, 2002-347-6111-221 and the Korean Genome Analysis Project 4845-301) as well as by National Research Foundation of Korea Grant funded by the Korean Government (2009-0070038).

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