Journal List > Tuberc Respir Dis > v.65(6) > 1001318

Park, Kim, Yoon, Kyung, An, Lee, Park, and Jeong: Particulate Matter 10 from Asian Dust Storms Induces the Expression of Reactive Oxygen Species, NF-κB, TGF-β and Fibronectin in WI-26 VA4 Epithelial Cells

Abstract

Background

Particulate matter may be toxic to human tissue. Ambient air particulate matter ≤10µm in aerodynamic size (PM10), which changes under different environmental conditions, is a complex mixture of organic and inorganic compounds. The Asian dust event caused by meteorological phenomena can also spread unique particulate matter in affected areas. We evaluated production of ROS, TGF-β, fibronectin, and NFκB by exposing normal epithelial cells to Asian dust particulate matter.

Methods

Bronchial epithelial cells were exposed to 0, 50, 100µg/ml of a suspension of PM10 for 24 h. ROS were detected by measurement of DCF release from DCF-DA by FACScan. TGF-β, fibronectin, and NFκB were detected by western blotting.

Results

PM10 exposure increased the expression of TGF-β, fibronectin, and NFκB. ROS production and TGF-βlevels were significantly higher with 50 or 100µg/ml PM10. Fibronectin and NFκB production were significantly higher after 100µg/ml of PM10.

Conclusion

PM10 from Asian dust particles might have fibrotic potential in bronchial epithelial cells via ROS induction after PM10 exposure.

Figures and Tables

Figure 1
(A) ROS expression in WI26-VA4 cells after 24 hours. WI 26 cells were exposed to 0 to 100µg/ml of a suspension of ambient particulate matter with a diameter of less than 10µm (PM10). (B) ROS expression between control and NAC in WI26-VA4 cells. The ROS levels were increased after exposed to PM10, compared to those in NAC and control. ROS expression increased after exposure to PM10 in a dose-dependent manner (*p<0.05, p<0.1).
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Figure 2
NF-κB expression in WI- 26 VA4 cells after 24 hours incubation with PM10. The NF-κB Levels were increased after exposed to PM10, compared to those in NAC and control. NFκB expression increased after 24 hours of exposure to PM10 in a dose-dependent manner.
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Figure 3
TGF-β expression in WI- 26 VA4 cells after 24 hours incubation with PM10. The TGF-β levels were increased after exposed to PM10, compared to those in NAC and control. TGF-β expression increased after 24 hours of exposure to PM10 in a dose-dependent manner (*p<0.05).
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Figure 4
Fibronectin expression in WI-26 VA4 cells after 24 hours incubation with PM10. The fibronectin levels were increased after exposed to PM10, compared to those in NAC and control. fibronectin expression increased after 24 hours of exposure to PM10 in a dose-dependent manner.
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Table 1
ROS expression in WI- 26 VA4 cells after 24 hours incubation with PM10
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Table 2
ROS expression between PM10 and PM10 plus NAC in WI-26 VA4 cells incubation with PM10
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Table 3
TGF-β expression in WI-26 VA4 cells after 24 hours incubation with PM10
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Table 4
TGF-β expression between PM10 and PM10 plus NAC in WI-26 VA4 cells incubation with PM10
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Table 5
Fibronectin expression in WI-26 VA4 cells after 24 hours incubation with PM10
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