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Abstract
Purpose
To investigate the efficacy of an amniotic membrane contact lens on corneal epithelial wound healing.
Methods
We made a model with a corneal epithelial wound by applying 6 mm round filter paper soaked with 1 N NaOHonto the central cornea in 24 eyes of 12 rabbits. The rabbits were divided into three groups: AMCL (amniotic membrane contact lens), T-AMT (temporary amniotic membrane transplantation) and the control group. We evaluated corneal wound healing every postoperative day using a digital photo slitlamp and fluorescein dye. The corneas were harvested for histopathologic studies after seven days and analyzed with hematoxylin-eosin (H & E) stain and TUNEL staining.
Results
The average wound healing time was similar between the amniotic membrane contact lens and the temporary amniotic membrane transplantation group. The number of the infiltrated PMNs (polymorphonuclear cells) was 8.8±2.58, 8.6±2.19 and 48.6±7.12 in the AMCL, T-AMT and control groups, respectively. Apoptotic keratocytes were 3.8±1.1, 3.6±1.09 and 23.2±5.06 in the AMCL, T-AMT and control groups, respectively. In the AMCL and T-AMT groups, the number of infiltrated PMNs and apoptotic keratocytes were significantly less than those the control group (p<0.05). There were not significant differences in the number of PMNs and apoptotic cells in the AMCL and the T-AMT groups.
References
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Figure 2.
Photograph of fluorescein dye staining shows corneal epithelial defect after alkali burn (A). Transplanted amniotic membrane contact lens (AMCL) is in place (B). Temporarily transplantated amniotic membrane (T-AMT) in place (C).
Figure 3.
Histopathologic findings of rabbit cornea with hematoxylin and eosin staining. Original magnification; ×200. Mild infiltration of inflammatory cells was detected in AMCL and T-AMT group (A and B). Infiltration of many inflammatory cells was found in the stroma in the control group (C). No definite differences are found between AMCL and T-AMT group.
Figure 4.
Comparison of PMNs infiltration in the stroma. Infiltration of many inflammatory cells was detected in the control group. There was no significant difference between AMCL and T-AMT group (* p<0.05). PMNs= polymorphonuclear cells; HPF=high power field.
Figure 5.
Histopathologic findings of rabbit cornea with TUNEL stain. Original magnification, ×200. The lesser TUNEL positive cells were detected in the anterior corneal stroma in AMCL and T-AMT group (A and B). More TUNEL positive cells were seen in the control group (C). There was no significant difference in the number of apoptotic cells between AMCL and T-AMT group.
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