Journal List > J Nutr Health > v.49(2) > 1081477

Lee, Kim, Hwang, and Cho: Dietary effect of green tea extract on epidermal levels of skin pH related factors, lactate dehydrogenase protein expression and activity in UV-irradiated hairless mice

Abstract

Purpose

Skin pH, an indicator of skin health, is maintained by various organic factors, which include lactate, free amino acid (FAA), and free fatty acid (FFA). As skin ages or with illness, skin pH becomes less acidic, and functional food has been developed to maintain the acidic pH of skin. In this study, we determined the dietary effect of green tea extract (GTE) on skin pH of photo-aged mice, as measured by epidermal levels of lactate, FAA, and FFA. The protein expression and activity of lactate dehydrogenase (LDH), an enzyme of pyruvate reduction for lactate generation, was further determined.

Methods

Albino hairless mice were fed a control diet (group UV+) or a diet with 1% GTE (group GTE) in parallel with UV irradiation for 10 weeks. A normal control group was fed a control diet without UV irradiation for 10 weeks (group UV-).

Results

Skin pH was higher (less acidic) in group UV+ than in group UV-. In parallel, epidermal levels of lactate and FFA, as well as of LDH protein expression and activity, were reduced in group UV+. Dietary supplementation of GTE (group GTE) reduced skin pH to similar to the level of group UV-, and inversely increased epidermal levels of lactate, LDH protein expression and activity, but not of FFA. Although epidermal levels of FAA were similar in groups UV- and UV+, it was increased in group GTE to a level higher than in group UV-. In further analysis of major FFA, epidermal levels of palmitic acid [16:0], oleic acid [18:1(n-9)], and linoleic acid [18:2(n-6), but not of stearic acid [18:0] in group GTE were similar to or lower than those in group UV+.

Conclusion

Dietary GTE normalized skin pH with increased levels of lactate and FAA, as well as with increased protein expression and activity of LDH in the epidermis of UVB irradiated hairless mice.

Figures and Tables

Fig. 1

Effect of UV irradiation and dietary green tea extract on skin pH. Groups UV- and UV, hairless mice fed a control diet without (group UV-) or with UV irradiation (group UV+) for 10 weeks; Group GTE, hairless mice fed a diet containing 1.0% green tea extract (GTE) in parallel with UV irradiation for 10 weeks. Values are mean ± SEM (n = 5). Values without a common letter are significantly different (p < 0.05) using one-way ANOVA and Duncan's multiple range test.

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Fig. 2

Effect of UV irradiation and dietary green tea extract on lactate dehydrogenase (LDH) protein expression and activity in the epidermis of mice. Groups UV- and UV+, hairless mice fed a control diet without (group UV-) or with UV irradiation (group UV+) for 10 weeks; Group GTE, hairless mice fed a diet containing 1.0% green tea extract (GTE) in parallel with UV irradiation for 10 weeks. (A) Representative expressions of LDH and actin in the epidermis of groups. (B) Signal intensities from multiple experiments of A were quantified and the integrated areas were normalized to the corresponding value of actin (the loading control). Values are mean ± SEM (n = 5). (C) The activity of LDH was determined in the epidermis of group by LDH activity colorimetric assay kit based on NADH generation in epidermis of groups. Values are mean ± SEM (n = 5). Values without a common letter are significantly different (p < 0.05) using one-way ANOVA and Duncan's multiple range test.

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Table 1

Diet composition and UV irradiation of experimental groups (g/kg)

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1) Groups UV- and UV+, hairless mice fed a control diet without (group UV-) or with (group UV+) UV irradiation for 10 weeks; Group GTE, hairless mice fed a diet containing 1.0% green tea extract (GTE) in parallel with UV irradiation for 10 weeks 2) GTE: green tea extract 3) AIN-93 vitamin mix #310025 (Dyets Inc., Bethlehem, PA) 4) AIN-93G salt mix #210025 (Dyets Inc., Bethlehem, PA) 5) Casein (nitrogen × 6.25), 870 g/kg

Table 2

Effect of UV irradiation and dietary green tea extract on epidermal levels of lactate, total free amino acids and total free fatty acids

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1) Groups UV- and UV+, hairless mice fed a control diet without (group UV-) or with UV irradiation (group UV+) for 10 weeks; Group GTE, hairless mice fed a diet containing 1.0% green tea extract (GTE) in parallel with UV irradiation for 10 weeks. 2) Data are mean ± SEM (n = 5). Values without a common letter are significantly different (p < 0.05) using one-way ANOVA and Duncan's multiple range test.

*p < 0.05 versus group UV+ by non-paired student's t-test

Table 3

Effect of UV irradiation and dietary green tea extract on epidermal levels of major free fatty acids

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1) FFA: free fatty acid 2) Groups UV- and UV+, hairless mice fed a control diet without (group UV-) or with UV irradiation (group UV+) for 10 weeks; Group GTE, hairless mice fed a diet containing 1.0% green tea extract (GTE) in parallel with UV irradiation for 10 weeks 3) Only major free fatty acids (FFA) are listed. 4) Data are mean ± SEM (n = 5). Values without a common letter are significantly different (p < 0.05) using one-way ANOVA and Duncan's multiple range test.

Notes

This study was supported by a grant of the Korean Health Technology R & D Project, Ministry of Health & Welfare, Republic of Korea (Grant No. HN13C0076).

References

1. Rippke F, Schreiner V, Schwanitz HJ. The acidic milieu of the horny layer: new findings on the physiology and pathophysiology of skin pH. Am J Clin Dermatol. 2002; 3(4):261–272.
2. Nakagawa N, Sakai S, Matsumoto M, Yamada K, Nagano M, Yuki T, Sumida Y, Uchiwa H. Relationship between NMF (lactate and potassium) content and the physical properties of the stratum corneum in healthy subjects. J Invest Dermatol. 2004; 122(3):755–763.
crossref
3. Elias PM. Stratum corneum acidification: how and why. Exp Dermatol. 2015; 24(3):179–180.
crossref
4. Lambers H, Piessens S, Bloem A, Pronk H, Finkel P. Natural skin surface pH is on average below 5, which is beneficial for its resident flora. Int J Cosmet Sci. 2006; 28(5):359–370.
crossref
5. Ali SM, Yosipovitch G. Skin pH: from basic science to basic skin care. Acta Derm Venereol. 2013; 93(3):261–267.
crossref
6. Choi EH, Man MQ, Xu P, Xin S, Liu Z, Crumrine DA, Jiang YJ, Fluhr JW, Feingold KR, Elias PM, Mauro TM. Stratum corneum acidification is impaired in moderately aged human and murine skin. J Invest Dermatol. 2007; 127(12):2847–2856.
crossref
7. Rabe JH, Mamelak AJ, McElgunn PJ, Morison WL, Sauder DN. Photoaging: mechanisms and repair. J Am Acad Dermatol. 2006; 55(1):1–19.
crossref
8. El-Domyati M, Attia S, Saleh F, Brown D, Birk DE, Gasparro F, Ahmad H, Uitto J. Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin. Exp Dermatol. 2002; 11(5):398–405.
crossref
9. Bissett DL, Hannon DP, Orr TV. An animal model of solar-aged skin: histological, physical, and visible changes in UV-irradiated hairless mouse skin. Photochem Photobiol. 1987; 46(3):367–378.
crossref
10. Holleran WM, Uchida Y, Halkier-Sorensen L, Haratake A, Hara M, Epstein JH, Elias PM. Structural and biochemical basis for the UVB-induced alterations in epidermal barrier function. Photodermatol Photoimmunol Photomed. 1997; 13(4):117–128.
crossref
11. Sterenborg HJ, de Gruijl FR, van der Leun JC. UV-induced epidermal hyperplasia in hairless mice. Photodermatol. 1986; 3(4):206–214.
12. Sime S, Reeve VE. Protection from inflammation, immunosuppression and carcinogenesis induced by UV radiation in mice by topical Pycnogenol. Photochem Photobiol. 2004; 79(2):193–198.
crossref
13. Kang TH, Park HM, Kim YB, Kim H, Kim N, Do JH, Kang C, Cho Y, Kim SY. Effects of red ginseng extract on UVB irradiationinduced skin aging in hairless mice. J Ethnopharmacol. 2009; 123(3):446–451.
crossref
14. Pazyar N, Feily A, Kazerouni A. Green tea in dermatology. Skinmed. 2012; 10(6):352–355.
15. Vayalil PK, Mittal A, Hara Y, Elmets CA, Katiyar SK. Green tea polyphenols prevent ultraviolet light-induced oxidative damage and matrix metalloproteinases expression in mouse skin. J Invest Dermatol. 2004; 122(6):1480–1487.
crossref
16. Li YH, Wu Y, Wei HC, Xu YY, Jia LL, Chen J, Yang XS, Dong GH, Gao XH, Chen HD. Protective effects of green tea extracts on photoaging and photommunosuppression. Skin Res Technol. 2009; 15(3):338–345.
crossref
17. Min J, Lee Y, Han SM, Choi Y. Dietary effect of royal jelly supplementation on epidermal levels of hydration, filaggrins, free amino acids and the related enzyme expression in UV irradiated hairless mice. Korean J Nutr. 2013; 46(2):109–118.
crossref
18. Jeon SE, Choi-Kwon S, Park KA, Lee HJ, Park MS, Lee JH, Kwon SB, Park KC. Dietary supplementation of (+)-catechin protects against UVB-induced skin damage by modulating antioxidant enzyme activities. Photodermatol Photoimmunol Photomed. 2003; 19(5):235–241.
crossref
19. Takami S, Imai T, Hasumura M, Cho YM, Onose J, Hirose M. Evaluation of toxicity of green tea catechins with 90-day dietary administration to F344 rats. Food Chem Toxicol. 2008; 46(6):2224–2229.
crossref
20. Senadheera D, Krastel K, Mair R, Persadmehr A, Abranches J, Burne RA, Cvitkovitch DG. Inactivation of VicK affects acid production and acid survival of Streptococcus mutans. J Bacteriol. 2009; 191(20):6415–6424.
21. Nachat R, Méchin MC, Takahara H, Chavanas S, Charveron M, Serre G, Simon M. Peptidylarginine deiminase isoforms 1-3 are expressed in the epidermis and involved in the deimination of K1 and filaggrin. J Invest Dermatol. 2005; 124(2):384–393.
crossref
22. Hamanaka S, Hara M, Nishio H, Otsuka F, Suzuki A, Uchida Y. Human epidermal glucosylceramides are major precursors of stratum corneum ceramides. J Invest Dermatol. 2002; 119(2):416–423.
crossref
23. Uchida Y, Hara M, Nishio H, Sidransky E, Inoue S, Otsuka F, Suzuki A, Elias PM, Holleran WM, Hamanaka S. Epidermal sphingomyelins are precursors for selected stratum corneum ceramides. J Lipid Res. 2000; 41(12):2071–2082.
crossref
24. Tang W, Ziboh VA. Reversal of epidermal hyperproliferation in essential fatty acid deficient guinea pigs is accompanied by rapid generation of inositol triphosphate. Arch Dermatol Res. 1988; 280(5):286–292.
crossref
25. Takagi Y, Nakagawa H, Yaginuma T, Takema Y, Imokawa G. An accumulation of glucosylceramide in the stratum corneum due to attenuated activity of beta-glucocerebrosidase is associated with the early phase of UVB-induced alteration in cutaneous barrier function. Arch Dermatol Res. 2005; 297(1):18–25.
crossref
26. Beamer CA, Holian A. Silica suppresses Toll-like receptor ligandinduced dendritic cell activation. FASEB J. 2008; 22(6):2053–2063.
crossref
27. Lampe MA, Burlingame AL, Whitney J, Williams ML, Brown BE, Roitman E, Elias PM. Human stratum corneum lipids: characterization and regional variations. J Lipid Res. 1983; 24(2):120–130.
crossref
28. Cook HW. Fatty acid desaturation and chain elongation in eukaryotes. In : Vance DE, Vance JE, editors. Biochemistry of Lipids and Membranes. Menlo Park, CA: Benjamin/Cummings Pub. Co.;1985. p. 181–203.
29. Khan SG, Katiyar SK, Agarwal R, Mukhtar H. Enhancement of antioxidant and phase II enzymes by oral feeding of green tea polyphenols in drinking water to SKH-1 hairless mice: possible role in cancer chemoprevention. Cancer Res. 1992; 52(14):4050–4052.
30. Kim EJ, Jin XJ, Kim YK, Oh IK, Kim JE, Park CH, Chung JH. UV decreases the synthesis of free fatty acids and triglycerides in the epidermis of human skin in vivo, contributing to development of skin photoaging. J Dermatol Sci. 2010; 57(1):19–26.
crossref
31. Bhawan J, Andersen W, Lee J, Labadie R, Solares G. Photoaging versus intrinsic aging: a morphologic assessment of facial skin. J Cutan Pathol. 1995; 22(2):154–159.
crossref
32. Hsu S. Green tea and the skin. J Am Acad Dermatol. 2005; 52(6):1049–1059.
crossref
33. Kim DS, Park SH, Kwon SB, Li K, Youn SW, Park KC. (-)-Epigallocatechin-3-gallate and hinokitiol reduce melanin synthesis via decreased MITF production. Arch Pharm Res. 2004; 27(3):334–339.
34. Katiyar SK, Elmets CA. Green tea polyphenolic antioxidants and skin photoprotection (Review). Int J Oncol. 2001; 18(6):1307–1313.
crossref
35. Di Cerbo A, Laurino C, Palmieri B, Iannitti T. A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, hyaluronic acid and carbonylated proteins. J Photochem Photobiol B. 2015; 144:94–103.
36. Kim H, Park KH, Yeo JH, Lee KG, Jeong DH, Kim SH, Cho Y. Dietary effect of silk protein sericin or fibroin on plasma and epidermal amino acid concentration of NC/Nga mice. Korean J Nutr. 2006; 39(6):520–528.
37. Levin J, Maibach H. Human skin buffering capacity: an overview. Skin Res Technol. 2008; 14(2):121–126.
crossref
38. Ronquist G, Andersson A, Bendsoe N, Falck B. Human epidermal energy metabolism is functionally anaerobic. Exp Dermatol. 2003; 12(5):572–579.
crossref
39. Lewis C Jr, Schmitt M, Hershey FB. Heterogeneity of lactic dehydrogenase of human skin. J Invest Dermatol. 1967; 48(3):221–225.
crossref
40. Huang D, Jungmann RA. Transcriptional regulation of the lactate dehydrogenase A subunit gene by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate. Mol Cell Endocrinol. 1995; 108(1-2):87–94.
crossref
41. Levites Y, Amit T, Mandel S, Youdim MB. Neuroprotection and neurorescue against Aβ toxicity and PKC-dependent release of nonamyloidogenic soluble precursor protein by green tea polyphenol (-)-epigallocatechin-3-gallate. FASEB J. 2003; 17(8):952–954.
crossref
42. Yosipovitch G, Tur E, Morduchowicz G, Boner G. Skin surface pH, moisture, and pruritus in haemodialysis patients. Nephrol Dial Transplant. 1993; 8(10):1129–1132.
43. Scott IR, Harding CR, Barrett JG. Histidine-rich protein of the keratohyalin granules: source of the free amino acids, urocanic acid and pyrrolidone carboxylic acid in the stratum corneum. Biochim Biophys Acta. 1982; 719(1):110–117.
44. Rawlings AV, Scott IR, Harding CR, Bowser PA. Stratum corneum moisturization at the molecular level. J Invest Dermatol. 1994; 103(5):731–741.
crossref
45. Thulin CD, Walsh KA. Identification of the amino terminus of human filaggrin using differential LC/MS techniques: implications for profilaggrin processing. Biochemistry. 1995; 34(27):8687–8692.
crossref
46. McGrath JA, Uitto J. The filaggrin story: novel insights into skinbarrier function and disease. Trends Mol Med. 2008; 14(1):20–27.
crossref
47. Hachem JP, Crumrine D, Fluhr J, Brown BE, Feingold KR, Elias PM. pH directly regulates epidermal permeability barrier homeostasis, and stratum corneum integrity/cohesion. J Invest Dermatol. 2003; 121(2):345–353.
crossref
48. Tyrrell RM. Activation of mammalian gene expression by the UV component of sunlight--from models to reality. Bioessays. 1996; 18(2):139–148.
49. Wang X, Song KS, Guo QX, Tian WX. The galloyl moiety of green tea catechins is the critical structural feature to inhibit fattyacid synthase. Biochem Pharmacol. 2003; 66(10):2039–2047.
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