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Lee, Song, Kim, Cha, and Uhm: Comparison of Retinal Nerve Fiber Layer Thickness Measured by Spectral-Domain and Time-Domain Optical Coherence Tomography
Original Article
Journal of the Korean Ophthalmological Society

Journal of the Korean Ophthalmological Society 2012; 53(1): 103-110.

Published online: 16 January 2012

DOI: https://doi.org/10.3341/jkos.2012.53.1.103

Comparison of Retinal Nerve Fiber Layer Thickness Measured by Spectral-Domain and Time-Domain Optical Coherence Tomography

Juhyang Lee, MD, In Seok Song, MD, Yu-Jeong Kim, MD, Yong Jae Cha, MD, Ki Bang Uhm, MD, PhD

Department of Ophthalmology, Hanyang University College of Medicine, Seoul, Korea.

Address reprint requests to Ki Bang Uhm, MD, PhD. Department of Ophthalmology, Hanyang University Hospital, #222 Wangsimni-ro, Seongdong-gu, Seoul 133-792, Korea. Tel: 82-2-2290-8570, Fax: 82-2-2291-8517, KBUhm@hanyang.ac.kr

Received 15 April 2011       Accepted 29 October 2011

Copyright © 2012 The Korean Ophthalmological Society

Abstract

Purpose

To compare retinal nerve fiber layer (RNFL) thickness measured by time domain (Stratus) and spectral domain (3D) optical coherence tomography (OCT).

Methods

Sixty-nine normal subjects and 92 glaucoma patients were included in the present study. Peripapillary RNFL thickness was measured with Stratus fast RNFL scan and 3D optic disc cube scan on the same day. The relationship between RNFL thickness measurements of the two OCTs were evaluated using a Pearson's correlation analysis. A Bland-Altman plot was used for the comparison of Stratus-OCT and 3D-OCT.

Results

In all subjects, average, superior, and inferior quadrant RNFL thicknesses measured by Stratus-OCT were thicker than those measured by 3D-OCT. In contrast, temporal and nasal quadrant RNFL thicknesses measured by 3D-OCT were thicker than those measured by Stratus-OCT. Although there were absolute value differences in RNFL thicknesses, high correlation was observed between Stratus-OCT and 3D-OCT (average: r = 0.897, temporal quadrant: r = 0.728, superior quadrant: r = 0.811, nasal quadrant: r = 0.678, and inferior quadrant: r = 0.905). Bland-Altman plots showed that Stratus-OCT values were greater than 3D-OCT values with relatively high RNFL thickness and the opposite with low RNFL thickness.

Conclusions

For thicker RNFL, Stratus-OCT measurements tend to be greater than 3D-OCT, while for thinner RNFL, 3D-OCT measurements tend to be greater than Stratus-OCT. This difference must be taken into account if comparing measurements made with a Stratus-OCT to the measurements of a 3D-OCT.

Keywords: Glaucoma, Retinal nerve fiber layer, Spectral domain optical coherence tomography

Figures and Tables

Figure 1
3D-OCT 1000 analysis report. An optic disc cube scan protocol was used to measure the RNFL thickness in a 6 × 6 mm2 area consisting of 512 × 128 axial scans (pixels) at the optic disc region. The average, quadrant, and clock-hour peripapillary RNFL thickness measurements were reported using the same categorical classification (in white or green = within normal limits, yellow = borderline, red = outside normal limits) as in the Stratus OCT.
jkos-53-103-g001
Figure 2
Bland-Altman plots of agreement between Stratus-OCT and 3D-OCT for the average (A) (The linear regression line equation is y = 0.185x - 14.622, r = 0.372, p < 0.001), temporal (B) (y = 0.123x - 10.587, r = 0.153, p = 0.052), superior (C) (y = 0.186x - 15.644, r = 0.279, p < 0.001), nasal (D) (y = 0.115x - 11.952, r = 0.130, p = 0.100), and inferior quadrant (E) (y = 0.275x - 21.499, r = 0.530, p < 0.001). The difference between both instruments is plotted against the average of both devices. Bold solid line is the mean of the average 3D-OCT and Stratus-OCT values. The dotted horizontal lines are ± 1.96 × SD confidence interval (CI) (i.e. 95% of the points should fall within these lines).
jkos-53-103-g002
Figure 3
Scatter plots showing the relationship between Stratus-OCT and 3D-OCT retinal nerve fiber layer thickness (RNFLT) for the inferior quadrant (A) and average (B).
jkos-53-103-g003
Table 1
Subject characteristics
jkos-53-103-i001

Data are expressed as mean ± standard deviation.

*By unpaired t-test; †By Chi-square test; ‡Measured using the Heidelberg Retina Tomograph II (version 3.04, Heidelberg Engineering, Heidelberg, Germany).

Table 2
Mean (95% confidence interval) average and quadrant-wise retinal nerve fiber layer thickness and correlation between Stratus-OCT and 3D-OCT in the 69 normal subjects and 92 glaucoma patients
jkos-53-103-i002

*p = correlation; †p = paired t-test

Table 3
Comparison of retinal nerve fiber layer thickness measured by Stratus-OCT and 3D-OCT
jkos-53-103-i003

Data are expressed as mean ± standard deviation.

By paired t-test

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