Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells

Jae Woo Kim; Sin Hoo Kim; Jae Hyung Lee

doi:10.3341/jkos.2008.49.10.1665

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Woo Kim, Hoo Kim, and Hyung Lee: Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells

Original Article

J Korean Ophthalmol Soc 2008;49(10):1665-1664.

Published online: 7 September 2008

DOI: https://doi.org/10.3341/jkos.2008.49.10.1665

Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells

Jae Woo Kim, M.D, Sin Hoo Kim, M.D, Jae Hyung Lee, M.D

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Koera

Address reprint requests to J ae Woo Kim, M.D. Department of Ophthalmology, Catholic University of Daegu College of Medicine #3056-6 Daemyeung-4-dong, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 10 July 2008 Accepted 9 May 2008

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence.

Methods

Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-β-gal assays, respectively.

Results

Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence.

Conclusions

Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma.

Keywords: Ascorbic acid, Hydrogen peroxide, Oxidative stress, Senescence, Trabecular meshwork cells

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Figure 1.

Effect of hydrogen-peroxide-induced oxidative stress on the production of NO in cultured trabecular meshwork cells. Hydrogen peroxide inhibited NO production significnatly, which was abolished by co-exposed L-ascorbic acid (LAA) (* p<0.05).

Figure 2.

Effect of hydrogen peroxide on the survival of trabecular meshwork cells. Hydrogen peroxide decreased cellular survival significantly in a dose dependent manner. Co-exposed 0.1 mM L-ascorbic acid did not affect on the survival (* p<0.05).

Figure 3.

Photograph of SA-β-gal positive senescent trabecular meshwork cells (blue color) after exposed to 0.05 mM of hydrogen peroxide. Original magnification ×100.

Figure 4.

Effect of hydrogen peroxide on the induction of cellular senescence in cultured trabecular meshwork cells. Hydrogen peroxide increased cellular senescence significantly, which was not abolished by co-exposed 0.1 mM L-ascorbic acid (* p<0.05).

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