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       Journal List > J Korean Med Sci > v.23(4); Aug 2008
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J Korean Med Sci. 2008 Aug;23(4):667-673.
Published online 2008 August 25.  doi: 10.3346/jkms.2008.23.4.667.
Copyright © 2008 The Korean Academy of Medical Sciences
Cyclin-Dependent Kinase Inhibitor p27Kip1 Controls Growth and Cell Cycle Progression in Human Uterine Leiomyoma
Sabarish Ramachandran, Kun-Young Kwon,* So-Jin Shin, Sang-Hoon Kwon, Soon-Do Cha, Insoo Bae,† and Chi-Heum Cho
Department of Obstetrics and Gynecology, Keimyung University, School of Medicine, Daegu, Korea.
*Department of Pathology, Keimyung University, School of Medicine, Daegu, Korea.
†Department of Oncology, Lombardi Cancer Center, Georgetown University, USA.

Address for correspondence: Chi-Heum Cho, M.D. Department of Obstetrics and Gynecology, Keimyung University, School of Medicine, 194 Dongsan-dong, Jung-gu, Daegu 700-712, Korea. Tel: +82.53-250-7518, Fax: +82.53-250-7599, Email: c0035@dsmc.or.kr
Received August 11, 2007; Accepted December 04, 2007.

Abstract

The molecular mechanism of the cell-cycle machinery in uterine leiomyoma has not yet been fully elucidated. Among the various types of cell-cycle regulators, p27Kip1 (p27) is considered to be a potent tumor suppressor. To provide further molecular basis for understanding the progression of uterine leiomyoma, our objective was to evaluate the expression level of p27 in normal myometrium and uterine leiomyoma tissue and its effect on cytogenic growth. Western blot analysis, real-time polymerase chain reaction (PCR) and immunohistochemical staining revealed that p27 protein and messenger RNA were down-regulated in uterine leiomyoma tissue and cultured cells compared to normal myometerium. Full-length human p27 cDNA was transferred using a replication-deficient recombinant adenoviral vector (Ad.p27) into uterine leiomyoma cells and evaluated the effect on cell proliferation. Transfection of Ad.p27 into uterine leiomyoma cells resulted in the induction of apoptosis, reduction in viability and proliferation of uterine leiomyoma cells. Our results suggest a new paradigm that down-regulated p27 protein expression is the possible underlying mechanism for the growth of uterine leiomyoma and over-expression of p27 induces cell death. This study provides better understanding of the control exerted by p27 in regulating growth and disease progression of uterine leiomyoma.

Keywords: Uterus, Leiomyoma, Cyclin-Dependent Kinase Inhibitor p27, Apoptosis.
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